使用流通解吸和在线固相萃取-串联质谱法探索自动干血斑分析的新概念。
Exploration of a new concept for automated dried blood spot analysis using flow-through desorption and online SPE-MS/MS.
作者信息
Ooms Jan Albert, Knegt Lena, Koster Emile Hermannus Maarten
机构信息
Spark Holland B.V., Pieter de Keyserstraat 8, Emmen, The Netherlands.
出版信息
Bioanalysis. 2011 Oct;3(20):2311-20. doi: 10.4155/bio.11.214.
BACKGROUND
A new concept for simple and generic automation of dried blood spot (DBS) analysis is evaluated. Flow-through desorption of the blood spot is coupled online to SPE and MS/MS without using a LC column. Blood, spiked with a mixture of test compounds is spotted on paper, dried and then desorbed by means of a prototype clamping device.
RESULTS
Conditions for extraction and chromatography on a single SPE cartridge were optimized with respect to clean-up, separation and ionization suppression. Addition of internal standard using loop-injection upstream of the clamped blood spot was examined and proved to be simple and reliable. Validation results for the 1-1000 ng/ml range are well within acceptance criteria for bioanalysis.
CONCLUSION
Results demonstrate feasibility of the DBS SPE-MS/MS concept for efficient automation of the entire DBS analysis workflow.
背景
对一种用于干血斑（DBS）分析的简单通用自动化新概念进行了评估。血斑的流通解吸在线连接到固相萃取（SPE）和串联质谱（MS/MS），无需使用液相色谱（LC）柱。将添加了测试化合物混合物的血液点样在滤纸上，干燥后通过原型夹紧装置进行解吸。
结果
针对净化、分离和电离抑制，对单个SPE小柱上的萃取和色谱条件进行了优化。研究了在夹紧血斑上游使用定量环进样添加内标，结果证明其简单可靠。1 - 1000 ng/ml范围内的验证结果完全符合生物分析的验收标准。
结论
结果证明了DBS SPE-MS/MS概念对于整个DBS分析工作流程高效自动化的可行性。
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