Asthma Center, Beijing Children's Hospital, Capital Medical University, 56 South Lishi Road, Beijing, PR China.
Int Arch Allergy Immunol. 2010;153(1):19-26. doi: 10.1159/000301575. Epub 2010 Mar 30.
Regulatory T cells and immunosuppressive cytokines, such as IL-10 and TGF-beta(1), may have a role in clinically effective allergen-specific immunotherapy. IL-10-secreting regulatory T cells have emerged as potential mediators of immune tolerance in numerous murine models of immunopathology. The aim of this study was to evaluate the frequency and function of regulatory T cells in the response to house dust mite (HDM) immunotherapy.
PBMCs were isolated from 27 HDM-allergic asthmatic children who underwent immunotherapy for 1.5-2 years (SIT group) and from 27 matched treated asthmatic children allergic to HDM (asthma group). After 48 h of in vitro stimulation with HDM extracts, regulatory T cells were measured by flow cytometry. Production of IL-4, IFN-gamma and TGF-beta(1 )in supernatants from allergen-stimulated cultures and the PBMC proliferations were measured by ELISA. Sera were tested for allergen-specific IgE using the ImmunoCAP 100 assay.
Patients undergoing immunotherapy produced significantly more IL-10 and showed a significant reduction in proliferation induced by HDM extract compared with the asthma group. In cultures stimulated with HDM extract, the amounts of IL-4 and TGF-beta were lower and the amounts of IFN-gamma were higher in the SIT group compared with the asthma group.
There is a functional, but quantitative, insufficiency of Treg cells in allergic asthmatic children, which was reversed in SIT-treated children. SIT can up-regulate the function of CD4(+)CD25(+)Foxp3(+) Treg cells.
调节性 T 细胞和免疫抑制细胞因子，如 IL-10 和 TGF-β（1），可能在临床上有效的过敏原特异性免疫治疗中发挥作用。在许多免疫病理学的鼠模型中，IL-10 分泌的调节性 T 细胞已成为免疫耐受的潜在介质。本研究旨在评估调节性 T 细胞在屋尘螨（HDM）免疫治疗反应中的频率和功能。
从 27 名接受 1.5-2 年免疫治疗的 HDM 过敏哮喘儿童（SIT 组）和 27 名接受 HDM 治疗的过敏哮喘儿童（哮喘组）中分离 PBMC。用 HDM 提取物体外刺激 48 小时后，通过流式细胞术测量调节性 T 细胞。通过 ELISA 测量过敏原刺激培养物上清液中 IL-4、IFN-γ和 TGF-β（1）的产生以及 PBMC 增殖。使用 ImmunoCAP 100 测定法检测血清中的过敏原特异性 IgE。
与哮喘组相比，接受免疫治疗的患者产生了更多的 IL-10，并且对 HDM 提取物诱导的增殖有明显的抑制作用。与哮喘组相比，在 HDM 提取物刺激的培养物中，SIT 组的 IL-4 和 TGF-β 量较低，IFN-γ量较高。
在过敏性哮喘儿童中存在功能但数量不足的 Treg 细胞，在 SIT 治疗的儿童中得到逆转。SIT 可以上调 CD4（+）CD25（+）Foxp3（+）Treg 细胞的功能。
