Okudan N, Revan S, Balci S S, Belviranli M, Pepe H, Gökbel H
Department of Physiology, Selcuk University, Konya, Turkey.
Bratisl Lek Listy. 2012;113(7):393-9. doi: 10.4149/bll_2012_089.
This study examined the combined effects of swimming training and coenzyme Q10 (CoQ10) supplementation on exhaustive exercise-induced oxidative stress in rat heart. The study was carried out with 4-month-old young adult male Wistar rats. Sixty four rats were divided mainly into two groups: trained and control. Each group was further divided into four subgroups: rest, exhausted, rest with CoQ10, exhausted with CoQ10. The training program consisted of swimming one hour each day, five days a week, for six weeks. At the end of sixth week, rats in exhausted exercise group were forced to swim until exhaustion and then they were immediately sacrificed, while rats in rest group were sacrificed at rest. Training alone or in combination with CoQ10 supplementation reduced to increasing MDA levels due to exhaustive exercise in rat heart (p<0.05). The trained-rest with CoQ10 group showed lower 8-OHdG levels than the control-rest with CoQ10 group. Exhaustive exercise effect was significant on SOD activity. Exhaustive exercise increased GSH levels in control groups while decreased GSH levels in training groups (p<0.05). In conclusion, the results suggest that CoQ10 supplementation combined with training may inhibit lipid peroxidation and DNA damage in the heart tissue. Also, it can be said that SOD activity and GSH levels were not influenced by CoQ10 supplementation (Fig. 4, Tab. 1, Ref. 69).
本研究考察了游泳训练和补充辅酶Q10（CoQ10）对大鼠心脏力竭运动诱导的氧化应激的联合作用。该研究以4月龄的成年雄性Wistar大鼠进行。64只大鼠主要分为两组：训练组和对照组。每组进一步分为四个亚组：休息组、力竭组、CoQ10休息组、CoQ10力竭组。训练方案为每天游泳1小时，每周5天，共6周。在第6周结束时，力竭运动组的大鼠被迫游泳直至力竭，然后立即处死，而休息组的大鼠在休息时处死。单独训练或与补充CoQ10相结合可降低大鼠心脏因力竭运动而升高的丙二醛（MDA）水平（p<0.05）。训练-CoQ10休息组的8-羟基脱氧鸟苷（8-OHdG）水平低于对照-CoQ10休息组。力竭运动对超氧化物歧化酶（SOD）活性有显著影响。力竭运动使对照组的谷胱甘肽（GSH）水平升高，而使训练组的GSH水平降低（p<0.05）。总之，结果表明补充CoQ10与训练相结合可能抑制心脏组织中的脂质过氧化和DNA损伤。此外，可以说SOD活性和GSH水平不受补充CoQ10的影响（图4，表1，参考文献69）。
