Baltaci A K, Mogulkoc R, Salbacak A, Celik I, Sivrikaya A
Selcuk University, Department of Physiology, Konya, Turkey.
Bratisl Lek Listy. 2012;113(7):400-3. doi: 10.4149/bll_2012_090.
The objective of the present study was to examine the effects of zinc supplementation on the oxidant damage in lung and liver tissues in rats exposed to a 50-Hz frequency magnetic field for 5 minutes every other day over a period of 6 months.
The study included 24 adult male Sprague-Dawley rats, which were divided into the three groups in equal numbers: Group 1, the control group (G1); Group 2, the group exposed to an electromagnetic field (G2); and Group 3, the group, which was exposed to an EMF and supplemented with zinc (G3). At the end of the 6-month procedures, the animals were decapitated to collect lung and liver tissue samples, in which MDA was analyzed using the "TBARS method (nmol/g/protein)", GSH by the "biuret method (mg/g/protein)" and zinc levels by atomic emission (µg/dl).
MDA levels in lung and liver tissues in G2 were higher than those in G1 and G3, and the levels in G3 were higher than those in G1 (p<0.01). As for GSH levels, G3 had GSH levels higher than G1 and G2, and G2 had GSH levels higher than G1 (p<0.01). Zinc values in the liver and lung tissues were the highest in the Group 3 and the lowest in the Group 2 (p<0.01).
Results of the study indicated that exposure to an electromagnetic field caused cellular damage in lung and liver tissues and zinc supplementation inhibited the inflicted cellular damage. Another important result of this study that needs emphasis was that exposure to an electromagnetic field led to a significant decrease in zinc levels in lung and liver tissues (Tab. 3, Ref. 23).
本研究的目的是检测在6个月的时间里，每隔一天让大鼠暴露于50赫兹频率的磁场中5分钟，补充锌对大鼠肺和肝组织氧化损伤的影响。
该研究包括24只成年雄性Sprague-Dawley大鼠，它们被平均分为三组：第1组，对照组（G1）；第2组，暴露于电磁场的组（G2）；第3组，暴露于电磁场并补充锌的组（G3）。在6个月的实验结束时，将动物断头以收集肺和肝组织样本，其中丙二醛（MDA）采用“硫代巴比妥酸反应物法（nmol/g/蛋白质）”进行分析，谷胱甘肽（GSH）采用“双缩脲法（mg/g/蛋白质）”进行分析，锌水平采用原子发射法（μg/dl）进行分析。
G2组肺和肝组织中的MDA水平高于G1组和G3组，且G3组的水平高于G1组（p<0.01）。至于GSH水平，G3组高于G1组和G2组，G2组高于G1组（p<0.01）。肝和肺组织中的锌含量在第3组最高，在第2组最低（p<0.01）。
研究结果表明，暴露于电磁场会导致肺和肝组织的细胞损伤，补充锌可抑制这种细胞损伤。本研究另一个需要强调的重要结果是，暴露于电磁场会导致肺和肝组织中的锌水平显著降低（表3，参考文献23）。
