Mackler B F, Withers J A, Woodson D L, Coker E, Herrin A, Friedman L, O'Neill P A
J Dent Res. 1979 Oct;58(10):1946-52. doi: 10.1177/00220345790580100101.
Various methodologies were examined for the isolation of inflammatory cells from diseased human gingiva. The best recovery of viable gingival lymphocytes (gMNC) was achieved by a method which combined initial collagenase digestion followed by gentle teasing with an 18-gauge needle.