胚胎分割可以增加囊胚的数量，但不能提高其质量。

Embryo splitting can increase the quantity but not the quality of blastocysts.

机构信息

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Kuo General Hospital, Tainan, Taiwan.

出版信息

Taiwan J Obstet Gynecol. 2012 Jun;51(2):236-9. doi: 10.1016/j.tjog.2012.04.012.

DOI:10.1016/j.tjog.2012.04.012

PMID:22795100

Abstract

OBJECTIVE

In this study, we investigated the developmental potential of single blastomeres that were obtained from 4-cell mice embryos that were split during the blastocyst stage.

MATERIALS AND METHODS

Imprinting Control Region (ICR) mice (age: 6-8 weeks), were superovulated and mated with a single fertile male of the same strain. We obtained 2-cell embryos that were then cultured in 4 groups (×4) with Human tubal fluid (HTF) supplemented with 12% fetal bovine serum. When these embryos reached the 4-cell stage, their zonae pellucidae were removed and every single blastomere was isolated by repeated pipetting with Ca/Mg(2+)-free medium. The isolated blastomeres (study group) and the intact embryos (control group) were then cultured to determine the blastocyst formation rate and quality.

RESULTS

We collected a total of 936 embryos from 524 morphologically intact, top-grade embryos in the 4-cell stage from 80 stimulated mice. We used 356 of these embryos to isolate the blastomeres. The remaining 168 embryos were cultured as controls. A total of 1312 single blastomeres were obtained and cultured in vitro. Among these, 620 blastocysts were harvested from the original embryos compared with 136 blastocysts that were harvested from the control group. The overall blastocyst formation rate was 174.2% (620 blastocysts from 356 embryos) for the study group compared with 81.5% (136 blastocysts from 168 embryos) for the control group. The study group was 43.3% (268 of 620) top-grade blastocysts compared with 91% (152 of 168) of the control group. Taken together, the percentage of top-grade blastocysts obtained per original embryo in the split group was 75.4% (174.2%×43.3%) compared with 74.2% (81.5%×91%) for the control group.

CONCLUSIONS

Embryo splitting can increase the number of blastocysts. However, the percentage of available top-grade blastocysts is the same compared with nonsplit embryos. Embryo splitting may not be a cost-effective technique for the generation of high-quality mouse blastocysts.

摘要

目的

在这项研究中，我们研究了从囊胚期分裂的 4 细胞期小鼠胚胎中获得的单个卵裂球的发育潜能。

材料和方法

ICR 小鼠（6-8 周龄）超数排卵并与同系的单个可育雄性交配。我们获得了 2 细胞胚胎，然后在 4 组（×4）中用含有 12%胎牛血清的人输卵管液（HTF）进行培养。当这些胚胎达到 4 细胞阶段时，去除它们的透明带并用无 Ca/Mg(2+)的培养基反复抽吸分离单个卵裂球。分离的卵裂球（研究组）和完整胚胎（对照组）然后进行培养，以确定囊胚形成率和质量。

结果

我们从 80 只接受刺激的小鼠中获得的 524 个形态完整的 4 细胞期优质胚胎中总共收集了 936 个胚胎。我们用其中的 356 个胚胎来分离卵裂球。其余的 168 个胚胎作为对照进行培养。总共获得了 1312 个单个卵裂球并在体外培养。其中，从原始胚胎中收获了 620 个囊胚，而从对照组中收获了 136 个囊胚。研究组的总囊胚形成率为 174.2%（356 个胚胎中的 620 个囊胚），而对照组为 81.5%（168 个胚胎中的 136 个囊胚）。研究组的优质囊胚率为 43.3%（620 个囊胚中的 268 个），而对照组为 91%（168 个囊胚中的 152 个）。总的来说，分裂组中每个原始胚胎获得的优质囊胚的百分比为 75.4%（174.2%×43.3%），而对照组为 74.2%（81.5%×91%）。