School of Pharmacy, Otago University, 18 Frederick Street, Dunedin 9016, New Zealand.
J Pharm Biomed Anal. 2012 Nov;70:456-61. doi: 10.1016/j.jpba.2012.06.024. Epub 2012 Jun 26.
The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6 ng/g. Secondly, MPH-spiked porcine brain tissue (450 mg/kg) was used to investigate drug concentration in larvae over a period of 72 h. After larvae had feed for 60 h, MPH was detected at 19.8±1.4 μg/g in the feeding larvae and at 3.5±0.1 μg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.
本研究旨在探讨利用蝇蛆（双翅目：丽蝇科）作为一种替代毒理学标本，用于检测精神药物模型药物哌醋甲酯（MPH）的潜在法医应用。通过液-液萃取，从生物基质（大鼠脑、血清和蝇蛆）中提取 MPH，回收率>80%，并用液相色谱-串联质谱（LC-MS/MS）定量。该 LC-MS/MS 测定法已验证可用于昆虫毒理学用途，并初步应用于经 MPH（20mg/kg）预给药的雄性 Sprague-Dawley 大鼠（n=6）。可在饲养于大鼠脑中的蝇蛆（n=15）中检测到 MPH（3.2±1.6ng/g）。其次，使用 MPH 掺杂的猪脑组织（450mg/kg）研究了在 72 小时内幼虫中的药物浓度。幼虫饲养 60 小时后，在取食幼虫中检测到 MPH 为 19.8±1.4μg/g，在 MPH 掺杂的猪脑组织中检测到 3.5±0.1μg/g。利用蝇蛆作为替代毒理学标本来检测碱性不稳定药物（如 MPH）可能具有优势。
