分子生物学对法医昆虫学的贡献。
The Contribution of Molecular Biology to Forensic Entomology.
作者信息
Scieuzo Carmen, Rinaldi Roberta, De Stefano Federica, Di Fazio Aldo, Falabella Patrizia
机构信息
Department of Basic and Applied Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Spinoff XFlies s.r.l, University of Basilicata, Via dell'Ateneo Lucano 10, 85100 Potenza, Italy.
出版信息
Insects. 2025 Jul 5;16(7):694. doi: 10.3390/insects16070694.
This review presents an in-depth analysis of the synergistic role of molecular biology in advancing forensic entomology. The study discusses how insects associated with decomposing bodies provide critical data for estimating the post-mortem interval (PMI), and how molecular techniques improve species identification and trace analysis. The manuscript examines DNA-based methods such as RAPD, RFLP, and mitochondrial sequencing, along with innovative applications like gene expression profiling and entomotoxicology analysis. Additionally, it presents real case studies illustrating how molecular data from insects can be used not only to estimate PMI but also to identify victims or suspects through human DNA retrieved from insect tissues. These advances confirm the fundamental role of molecular biology in strengthening the reliability and applicability of forensic entomology in legal contexts.
本综述深入分析了分子生物学在推进法医昆虫学发展中的协同作用。该研究讨论了与尸体分解相关的昆虫如何为估计死后间隔时间（PMI）提供关键数据，以及分子技术如何改进物种鉴定和痕量分析。该手稿研究了基于DNA的方法，如随机扩增多态性DNA（RAPD）、限制性片段长度多态性（RFLP）和线粒体测序，以及基因表达谱分析和昆虫毒理学分析等创新应用。此外，它还展示了实际案例研究，说明昆虫的分子数据不仅可用于估计PMI，还可通过从昆虫组织中提取的人类DNA识别受害者或嫌疑人。这些进展证实了分子生物学在加强法医昆虫学在法律背景下的可靠性和适用性方面的基础作用。
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