Department of Surgery, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, USA.
J Surg Res. 2013 May;181(2):355-8. doi: 10.1016/j.jss.2012.06.056. Epub 2012 Jul 6.
Patch arteriotomies are performed during many vascular procedures. Whereas synthetic materials are generally felt to be inappropriate for infected environments, the suitability of glutaraldehyde-treated bovine pericardium (GBP), a biologic material, in such instances is unknown. Our main objectives were to develop an animal model to study vascular prostheses while comparing the infectability of polyester (Dacron) and GBP in a topically infected environment.
Twenty-three pigs underwent transabdominal patch arteriotomy of the infrarenal aorta with either Dacron or GBP. The patches were inoculated with sterile saline (1 per group), Staphylococcus aureus 10(4) colony-forming units (CFUs) (4 per group), or S. aureus 10(5) CFUs (6 per group). At 3 wk, the animals were euthanized, and the patches were removed via a left retroperitoneal approach. Specimens were collected for microbiologic and histologic analysis.
One animal from each group inoculated with 10(5) CFUs died during the study period, and another died immediately postoperatively of an airway complication. All aortas were patent and without evidence of pseudoaneurysm formation. Gross abscesses were found in 4/6 Dacron and 5/6 GBP animals receiving 10(5) CFUs. Similarly, 4/6 animals implanted with Dacron and 5/6 animals implanted with GBP had positive tissue cultures. A histologic grading system of inflammation substantiated the culture results.
No significant difference exists between Dacron and GBP to resist bacterial infection at 3 wk. We have established a reproducible in vivo model to study arterial patch materials in a topically infected environment.
在许多血管手术中都会进行贴片血管切开术。虽然人们普遍认为合成材料不适合感染环境，但在这种情况下，戊二醛处理的牛心包（GBP）这种生物材料是否适用尚不清楚。我们的主要目的是开发一种动物模型来研究血管假体，并比较聚酯（涤纶）和 GBP 在局部感染环境中的感染能力。
23 头猪接受了经腹下腹主动脉贴片血管切开术，使用的贴片材料为涤纶或 GBP。每组各有 1 个贴片用无菌生理盐水（每组 1 个）、金黄色葡萄球菌 10（4）个菌落形成单位（CFU）（每组 4 个）或金黄色葡萄球菌 10（5）CFU（每组 6 个）进行接种。在 3 周时，通过左侧腹膜后入路处死动物，并取出贴片。采集标本进行微生物学和组织学分析。
每组中接种 10（5）CFU 的 1 只动物在研究期间死亡，另一只动物在术后立即死于气道并发症。所有主动脉均保持通畅，无假性动脉瘤形成的证据。在接受 10（5）CFU 的 4/6 只涤纶和 5/6 只 GBP 动物中发现了明显的脓肿。同样，植入涤纶的 4/6 只动物和植入 GBP 的 5/6 只动物的组织培养均为阳性。炎症的组织学分级系统证实了培养结果。
在 3 周时，涤纶和 GBP 在抵抗细菌感染方面没有显著差异。我们已经建立了一种可重复的体内模型，用于研究局部感染环境中的动脉贴片材料。
