State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Aug 1;902:96-107. doi: 10.1016/j.jchromb.2012.06.032. Epub 2012 Jul 1.
A novel chemometric-assisted high performance liquid chromatography method coupled with diode array detector (HPLC-DAD) was presented for the simultaneous determination of eleven antihypertensives from multiple classes in most concerned matrix systems. With the aid of second-order calibration which enables specific information of analytes to be well extracted, the heavily overlapping profiles between analytes and the coeluting interferences can be successfully separated and thus accurately quantified. A great advantage of the novel strategy lies in the fact that the analysis could be carried out with the same isocratic mobile phase (methanol/KH(2)PO(4): 58:42, v/v, pH 2.60) in a short time regardless of the changes of matrices, such as human serum, health product and Chinese patent medicine. Both qualitative and quantitative results indicate that the hybrid strategy that using HPLC-DAD coupled with second-order chemometric method would be a high performance approach for the purpose of simultaneously quantifying multiple classes of antihypertensives in complex systems. Additionally, the analytical strategy can potentially benefit drug monitoring in both therapeutic research and pharmaceutical quality control. Moreover, the accuracy and reliability of the proposed methodology has been evaluated using several statistical parameters such as root mean squared error of prediction (RMSEP), figures of merit (FOM) and reproducibility of inter-day analysis.
本文提出了一种新型的基于化学计量学辅助的高效液相色谱法与二极管阵列检测器（HPLC-DAD）联用，用于同时测定多种类别的抗高血压药物在最相关的基质系统中的含量。通过二阶校准，可以很好地提取分析物的特征信息，从而成功分离分析物之间的严重重叠峰和共洗脱干扰，实现准确的定量分析。该新策略的一个显著优点在于，无论基质如何变化，如人血清、保健品和中药，都可以在短时间内使用相同的等度流动相（甲醇/磷酸氢二钾：58:42，v/v，pH2.60）进行分析。定性和定量结果均表明，采用 HPLC-DAD 与二阶化学计量学方法相结合的混合策略，是一种用于同时定量复杂体系中多种类别的抗高血压药物的高性能方法。此外，该分析策略在治疗研究和药物质量控制中都可以用于药物监测，具有潜在的应用价值。此外，还使用了预测均方根误差（RMSEP）、度量指标（FOM）和日内分析重现性等几个统计参数来评估所提出方法的准确性和可靠性。
