Feng Chao, Cai Ya-Ling, Ruan Jin-Lan
College of Pharmacy, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan, PR China.
J Pharm Biomed Anal. 2008 Jun 9;47(2):442-7. doi: 10.1016/j.jpba.2008.01.019. Epub 2008 Jan 19.
A simple, reliable and accurate method for the simultaneous separation and determination of 10 active components (psoralen, isopsoralen, emodin, oleanolic acid, stachydrine hydrochloride, ammonium glycyrrhizinate, glycyrrhizinate, schizandrol, imperatorin and isoimperatorin) in traditional Chinese medicine "YIGONG" capsule was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with diode array detection. The chromatographic separation was performed on a Eurospher C(18) column (250 mm x 4.6 mm i.d. with 5.0 microm particle size) with a acetonitrile-water gradient containing 0.5% (v/v) aqueous phosphoric acid as mobile phase. Two detection wavelengths (210 and 250 nm) were utilized for the quantitative analysis due to the different UV spectra of these components. Good linear behaviors over the investigated concentration ranges were observed with the values of R(2) higher than 0.999 for all the analytes. The recoveries, measured at three concentration levels, varied from 95.0 to 105.3%. The validated method was successfully applied to the simultaneously determination of these active components in "YIGONG" capsule from different production batches.
采用反相高效液相色谱（RP-HPLC）结合二极管阵列检测，开发了一种简单、可靠且准确的方法，用于同时分离和测定中药“益宫”胶囊中的10种活性成分（补骨脂素、异补骨脂素、大黄素、齐墩果酸、盐酸水苏碱、甘草酸铵、甘草酸、五味子醇、欧前胡素和异欧前胡素）。色谱分离在Eurospher C(18)柱（250 mm×4.6 mm内径，粒径5.0μm）上进行，以含0.5%（v/v）磷酸水溶液的乙腈-水梯度作为流动相。由于这些成分的紫外光谱不同，定量分析采用了两个检测波长（210和250 nm）。在研究的浓度范围内观察到良好的线性关系，所有分析物的R(2)值均高于0.999。在三个浓度水平下测得的回收率为95.0%至105.3%。该验证方法成功应用于不同生产批次“益宫”胶囊中这些活性成分的同时测定。
