Briestanská J
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czechoslovakia.
Folia Biol (Praha). 1990;36(6):312-8.
The relation of cytoplasmic microtubules to intracellular transport was studied in temperature-sensitive (ts) secretory mutants of Saccharomyces cerevisiae at permissive and nonpermissive temperature using indirect immunofluorescence with monoclonal antibody TU-01 against alpha-tubulin. It was found that in the sec 1 mutant, which at 37 degrees C accumulated secretory vesicles and in which therefore transport of secretory material from secretory vesicles to cytoplasmic membrane was inhibited, cytoplasmic and in some cases nuclear microtubules were impaired. After 4 h of postcultivation at 24 degrees C the altered phenotype reverted to the original state, the cells began to divide, and were comparable with control. Use of the sec 1 mutant protoplasts suggested that the product, whose gene is mutated, is probably involved in microtubular polymerization. The sec 7 mutant, which accumulates the Golgi complex under nonpermissive conditions and in which the transfer of secretory material from the Golgi complex to the secretory vesicles is thus inhibited, showed no significant changes in the length or number of cytoplasmic microtubules. As a result of secretory product accumulation, the cytoplasmic microtubules were displaced towards the periphery in some cells.
利用抗α-微管蛋白的单克隆抗体TU-01，通过间接免疫荧光法，在允许温度和非允许温度下，研究了酿酒酵母温度敏感（ts）分泌突变体中细胞质微管与细胞内运输的关系。研究发现，在sec 1突变体中，37℃时积累分泌囊泡，因此分泌物质从分泌囊泡向细胞质膜的运输受到抑制，细胞质微管以及某些情况下的核微管受到损害。在24℃继代培养4小时后，改变的表型恢复到原始状态，细胞开始分裂，且与对照相当。对sec 1突变体原生质体的研究表明，其基因发生突变的产物可能参与微管聚合。sec 7突变体在非允许条件下积累高尔基体复合物，因此分泌物质从高尔基体复合物向分泌囊泡的转移受到抑制，其细胞质微管的长度或数量未显示出显著变化。由于分泌产物的积累，一些细胞中的细胞质微管向周边移位。
