Nihon University Graduate School of Dentistry at Matsudo, Crown Bridge Prosthodontics, Chiba 271-8587, Japan.
J Microbiol Methods. 2012 Oct;91(1):205-7. doi: 10.1016/j.mimet.2012.07.004. Epub 2012 Jul 11.
Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart Infusion (HI). Average growth recovery of R. dentocariosa on RDSM was 93.8% that of HI medium. Growth of other bacteria including Rothia mucilaginosa, Neisseria, Actinomyces, or streptococci was remarkably inhibited on the selective medium. Clinical efficacy was evaluated by the recovery of R. dentocariosa on RDSM from the stimulated saliva samples of 8 volunteers. R. dentocariosa was detected at 2.6 (range: 1.0-4.6) % to total bacteria of 8.0×10(7)CFU/ml on BHI in their oral cavities. The new selective medium, RDSM, was highly selective for R. dentocariosa and was useful for the isolation of R. dentocariosa from clinical samples.
罗氏菌（Rothia dentocariosa）是牙菌斑中的本土微生物菌群。为了研究口腔中的细菌种群，我们开发了一种新的选择性培养基，命名为 RDSM，用于分离罗氏菌（R. dentocariosa）。RDSM 通过在 Heart Infusion (HI) 中添加 0.5 μg/ml 的林可霉素、10 μg/ml 的黏菌素和 40%的蔗糖制备而成。罗氏菌（R. dentocariosa）在 RDSM 上的平均生长回收率为 HI 培养基的 93.8%。其他细菌，包括粘罗氏菌（Rothia mucilaginosa）、奈瑟氏菌（Neisseria）、放线菌（Actinomyces）或链球菌（streptococci）的生长在选择性培养基上受到明显抑制。通过从 8 名志愿者的刺激唾液样本中回收 RDSM 上的罗氏菌（R. dentocariosa）来评估临床疗效。在他们的口腔中，罗氏菌（R. dentocariosa）在 BHI 上的总细菌数为 8.0×10(7)CFU/ml，占比为 2.6%（范围：1.0-4.6%）。新的选择性培养基 RDSM 对罗氏菌（R. dentocariosa）具有高度选择性，可用于从临床样本中分离罗氏菌（R. dentocariosa）。
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