Bachtiar Boy M, Tahapary Dicky L, Fath Turmidzi, Theodorea Citra F, Haerani Natalina, Soeroso Yuniarti, Shahab Selvi Nafisa, Wildan Ardy, Runtu Fergie Marie Joe Grizella, Tadjoedin Fatimah Maria, Ayuningtyas Dewi, Amir Lisa, Bachtiar Endang W
Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Clinical Research Unit RSCM, Metabolic-Endocrine-Diabetes Division, Department Internal Medicine, Faculty of Medicine, Universitas Indonesia - Ciptomangunkusumo Hospital Metabolic Disorder, Cardiovascular, and Aging Research Cluster, Indonesian Medical Education and Research Institute, Jakarta, Indonesia.
Front Dent Med. 2025 Apr 22;6:1550936. doi: 10.3389/fdmed.2025.1550936. eCollection 2025.
Oral samples are widely used for studying oral microbiome in health and diseases. In this study, saliva and subgingival biofilm (SGB) samples obtained from patients with type2 diabetes (T2DM), without periodontitis (G1 group), with gingivitis (G2 group), and periodontitis (G3 group), were used to compare the abundance of (TM7), its host's bacteria (), periodontopathogen (Represented by ), and nitrate-reducing bacteria (represented by ). The gingival crevicular fluid were also used to analyze the transcription levels of interleukin-6 (IL-6) and C-reactive protein (CRP). Healthy individuals' oral samples served as a control, and the targeted bacteria and inflammatory indicators were detected and measured using real-time PCR. The results showed that in either sample, the abundance of TM7 and other targeted bacteria showed a similar profile. Notably, within participants with T2DM, the abundance of TM7 was similar in G1 and G2 groups, but significantly decreased in G3 group. With the exception of the SGB of the G3 group, the relationship between TM7 and its bacterial host was strongly positive across all evaluated samples. Furthermore, CRP had higher transcription levels than IL-6 across the entire group. Despite the fact that the G3 group showed an adverse relationship between TM7 and CRP, patients with T2DM generally showed a positive correlation between TM7 and IL-6/CRP, which was verified by a receiver operating curve.
口腔样本被广泛用于研究健康和疾病状态下的口腔微生物群。在本研究中，从2型糖尿病（T2DM）患者中获取唾液和龈下生物膜（SGB）样本，这些患者分别为无牙周炎（G1组）、患有牙龈炎（G2组）和患有牙周炎（G3组），用于比较嗜氨基酸菌属（TM7）、其宿主细菌、牙周病原体（以 表示）和硝酸盐还原菌（以 表示）的丰度。龈沟液也用于分析白细胞介素-6（IL-6）和C反应蛋白（CRP）的转录水平。健康个体的口腔样本作为对照，使用实时PCR检测和测量目标细菌及炎症指标。结果显示，在任何一种样本中，TM7和其他目标细菌的丰度呈现相似的分布。值得注意的是，在T2DM患者中，G1组和G2组中TM7的丰度相似，但在G3组中显著降低。除G3组的SGB外，在所有评估样本中，TM7与其细菌宿主之间的关系呈强正相关。此外，在整个组中CRP的转录水平高于IL-6。尽管G3组显示TM7与CRP之间存在负相关关系，但T2DM患者总体上TM7与IL-6/CRP之间呈正相关，这通过受试者工作特征曲线得到验证。
