Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China.
J Proteomics. 2012 Dec 21;77:68-86. doi: 10.1016/j.jprot.2012.07.005. Epub 2012 Jul 13.
Seed germination is an important stage in life cycle of higher plants. The germination processes and its associated loss of desiccation tolerance, however, are still poorly understood. In present study, pea seeds were used to study changes in embryonic axis proteome during germination by 2-DE and mass spectrometry. We identified a total of 139 protein spots showing a significant (>2-fold) change during germination. The results show that seed germination is not only the activation of a series of metabolic processes, but also involves reorganization of cellular structure and activation of protective systems. To uncouple the physiological processes of germination and its associated loss of desiccation tolerance, we used the fact that pea seeds have different desiccation tolerance when imbibed in water, CaCl(2) and methylviologen at the same germination stage. We compared the proteome amongst these seeds to identify the candidate proteins associated with the loss of desiccation tolerance and found a total of seven proteins - tubulin alpha-1 chain, seed biotin-containing protein SBP65, P54 protein, vicilin, vicilin-like antimicrobial peptides 2-3, convicilin and TCP-1/cpn60 chaperonin family protein. The metabolic function of these proteins indicates that seed desiccation tolerance is related to pathogen defense, protein conformation conservation and cell structure stabilization.
种子萌发是高等植物生命周期中的一个重要阶段。然而，萌发过程及其相关的干燥耐性丧失仍然知之甚少。在本研究中，使用豌豆种子通过 2-DE 和质谱法研究了萌发过程中胚胎轴蛋白质组的变化。我们共鉴定出了 139 个在萌发过程中发生显著 (>2 倍)变化的蛋白质斑点。结果表明，种子萌发不仅是一系列代谢过程的激活，还涉及细胞结构的重组和保护系统的激活。为了分离萌发及其相关干燥耐性丧失的生理过程，我们利用了这样一个事实，即在同一萌发阶段，豌豆种子在水、CaCl2 和甲紫精中吸胀时具有不同的干燥耐性。我们比较了这些种子之间的蛋白质组，以鉴定与干燥耐性丧失相关的候选蛋白，共鉴定出了 7 种蛋白 - 微管蛋白 α-1 链、种子生物素结合蛋白 SBP65、P54 蛋白、豆球蛋白、豆球蛋白样抗菌肽 2-3、浓缩素和 TCP-1/cpn60 伴侣蛋白家族蛋白。这些蛋白质的代谢功能表明，种子干燥耐性与病原体防御、蛋白质构象保守和细胞结构稳定有关。
