Bourgeois Michael, Jacquin Françoise, Savois Vincent, Sommerer Nicolas, Labas Valérie, Henry Céline, Burstin Judith
Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines, Institut National de la Recherche Agronomique, Bretenières, France.
Proteomics. 2009 Jan;9(2):254-71. doi: 10.1002/pmic.200700903.
Pea (Pisum sativum L.) is the most cultivated European pulse crop and the pea seeds mainly serve as a protein source for monogastric animals. Because the seed protein composition impacts on seed nutritional value, we aimed at identifying the determinants of its variability. This paper presents the first pea mature seed proteome reference map, which includes 156 identified proteins (http://www.inra.fr/legumbase/peaseedmap/). This map provides a fine dissection of the pea seed storage protein composition revealing a large diversity of storage proteins resulting both from gene diversity and post-translational processing. It gives new insights into the pea storage protein processing (especially 7S globulins) as a possible adaptation towards progressive mobilization of the proteins during germination. The nonstorage seed proteome revealed the presence of proteins involved in seed defense together with proteins preparing germination. The plasticity of the seed proteome was revealed for seeds produced in three successive years of cultivation, and 30% of the spots were affected by environmental variations. This work pinpoints seed proteins most affected by environment, highlighting new targets to stabilize storage protein composition that should be further analyzed.
豌豆（Pisum sativum L.）是欧洲种植最广泛的豆类作物，豌豆种子主要作为单胃动物的蛋白质来源。由于种子蛋白质组成会影响种子营养价值，我们旨在确定其变异性的决定因素。本文展示了首张豌豆成熟种子蛋白质组参考图谱，其中包括156种已鉴定的蛋白质（http://www.inra.fr/legumbase/peaseedmap/）。该图谱对豌豆种子贮藏蛋白组成进行了精细剖析，揭示了贮藏蛋白的多样性，这是由基因多样性和翻译后加工共同导致的。它为豌豆贮藏蛋白加工（尤其是7S球蛋白）提供了新的见解，这可能是对种子萌发过程中蛋白质逐步动员的一种适应。非贮藏种子蛋白质组揭示了参与种子防御的蛋白质以及为种子萌发做准备的蛋白质的存在。连续三年种植的豌豆种子蛋白质组的可塑性得以展现，30%的斑点受到环境变化的影响。这项工作确定了受环境影响最大的种子蛋白质，突出了稳定贮藏蛋白组成的新靶点，有待进一步分析。
