Zhao Shuo, Zou Hao, Jia Yingjie, Pan Xueqin, Huang Danfeng
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
School of Mechanical Engineering, Institute of Refrigeration and Cryogenics, Shanghai Jiao Tong University, Shanghai, China.
Front Plant Sci. 2022 Feb 10;13:824439. doi: 10.3389/fpls.2022.824439. eCollection 2022.
Asynchronized and non-uniform seed germination is causing obstacles to the large-scale cultivation of carrot ( L.). In the present study, the combination of high voltage electrostatic field treatment (EF) with hydropriming (HYD), namely hydro-electro hybrid priming (HEHP), significantly improved all germination indicators of carrot seeds, and the promoting effect was superior to that of the HYD treatment. A tandem mass tags (TMT)-based proteomic analysis identified 4,936 proteins from the seeds, and the maximum number of differentially abundant proteins (DAPs) appeared between CK and HEHP. KEGG analysis revealed that the upregulated DAPs were mainly enriched in the pathways related to protein synthesis and degradation such as "ribosome" and "proteasome," while the downregulated DAPs were mainly enriched in photosynthesis-related pathways. Furthermore, the maximum DAPs were annotated in carbohydrate metabolism. Some proteins identified as key enzymes of the glyoxylate cycle, the tricarboxylate cycle, glycolysis and the pentose phosphate pathway showed enhanced abundance in priming treatments. The activities of several key enzymes involved in carbohydrate metabolism were also enhanced by the priming treatments, especially the HEHP treatment. Real-time quantitative PCR (qRT-PCR) analysis revealed that the effect of priming is mainly reflected before sowing. In conclusion, the optimal effect of HEHP is to regulate the synthesis and degradation of proteins in seeds to meet the requirements of germination and initiate the utilization of seed storage reserves and respiratory metabolism. The present work expanded the understanding of the response mechanism of carrot seed germination to priming and the biological effects of high voltage electrostatic field.
异步且不均匀的种子萌发给胡萝卜（L.）的大规模种植带来了障碍。在本研究中，高压静电场处理（EF）与水引发（HYD）相结合，即水 - 电混合引发（HEHP），显著改善了胡萝卜种子的所有萌发指标，且促进效果优于HYD处理。基于串联质谱标签（TMT）的蛋白质组学分析从种子中鉴定出4936种蛋白质，差异丰富蛋白质（DAPs）数量最多的情况出现在CK和HEHP之间。KEGG分析表明，上调的DAPs主要富集在与蛋白质合成和降解相关的途径，如“核糖体”和“蛋白酶体”，而下调的DAPs主要富集在与光合作用相关的途径。此外，最大数量的DAPs注释在碳水化合物代谢中。一些被鉴定为乙醛酸循环、三羧酸循环、糖酵解和磷酸戊糖途径关键酶的蛋白质在引发处理中丰度增加。引发处理，尤其是HEHP处理，还增强了几种参与碳水化合物代谢的关键酶的活性。实时定量PCR（qRT-PCR）分析表明，引发的效果主要在播种前体现。总之，HEHP的最佳效果是调节种子中蛋白质的合成和降解，以满足萌发需求，并启动种子储存储备的利用和呼吸代谢。本研究扩展了对胡萝卜种子萌发对引发的响应机制以及高压静电场生物学效应的理解。
