Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
Virus Res. 2012 Oct;169(1):264-7. doi: 10.1016/j.virusres.2012.07.002. Epub 2012 Jul 13.
Although the functions of porcine respiratory and reproductive syndrome virus (PRRSV) proteins are increasingly understood, the roles of host factors in modifying infection are less well understood. Growing evidence places deubiquitination at the core of a multitude of regulatory processes, ranging from cell growth to innate immune response and health, such as cancer, degenerative and infectious diseases. This report provides further information on the functional role of the porcine ubiquitin-specific peptidase 18 (USP18) during innate immune responses to PRRSV. We have shown that constitutive overexpression of the porcine USP18 in MARC-145 cells restricts PRRSV growth, at least in part via early activation of NF-κB. Viral growth of PRRSV may be perturbed by increasing and decreasing nuclear translocation of p65 and p50, respectively. Our data highlight USP18 as a host restriction factor during innate immune response to PRRSV.
虽然猪繁殖与呼吸综合征病毒（PRRSV）蛋白的功能越来越被理解，但宿主因素在改变感染中的作用还不太清楚。越来越多的证据表明去泛素化在多种调节过程中处于核心地位，从细胞生长到先天免疫反应和健康，如癌症、退行性和传染病。本报告进一步提供了关于猪泛素特异性肽酶 18（USP18）在 PRRSV 先天免疫反应中的功能作用的信息。我们已经表明，在 MARC-145 细胞中组成性过表达猪 USP18 至少部分通过早期激活 NF-κB 来限制 PRRSV 的生长。PRRSV 的病毒生长可能会受到 p65 和 p50 核转位分别增加和减少的干扰。我们的数据强调了 USP18 作为 PRRSV 先天免疫反应中的宿主限制因子。
