Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Fish Shellfish Immunol. 2012 Sep;33(3):659-66. doi: 10.1016/j.fsi.2012.06.029. Epub 2012 Jul 13.
NF-κB transcription factors play central roles in many important physiological and pathological processes including innate immune responses. Here we report the cloning of an NF-κB transcription factor, PfRelish from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfRelish full-length cDNA is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. The deduced PfRelish contains a N-terminal RHD, a nucleus localization signal, an IκB-like domain with six ankyrin repeats and a death domain at the C-terminus, which is similar to class I NF-κB transcription factors. Comparison and phylogenetic analysis revealed that class I NF-κBs in mollusks including PfRelish might have most distant relationship to the arthropod Relish. Further expression analysis showed that PfRelish was apparently upregulated after Vibrio alginolyticus injection, which suggested that PfRelish was involved in the immune response to V. alginolyticus.
NF-κB 转录因子在许多重要的生理和病理过程中发挥核心作用，包括先天免疫反应。在这里，我们报道了珍珠贝（Pinctada fucata）NF-κB 转录因子 PfRelish 的克隆，珍珠贝是海水珍珠生产中最重要的双壳贝类之一。PfRelish 的全长 cDNA 为 3916bp，开放阅读框为 3558bp，编码一个推测的 1186 个氨基酸的蛋白质。推导的 PfRelish 包含一个 N 端 RHD、一个核定位信号、一个具有六个锚重复和 C 端死亡结构域的 IκB 样结构域，类似于 I 类 NF-κB 转录因子。比较和系统发育分析表明，包括 PfRelish 在内的软体动物中的 I 类 NF-κB 与节肢动物的 Relish 关系最疏远。进一步的表达分析表明，PfRelish 在弧菌（Vibrio alginolyticus）注射后明显上调，表明 PfRelish 参与了对 V. alginolyticus 的免疫反应。
