Qingdao Key Laboratory of Terahertz Technology, Department of Physics, School of Science, Shandong University of Science and Technology, Qingdao 266510, People's Republic of China.
Anal Biochem. 2012 Oct 1;429(1):42-4. doi: 10.1016/j.ab.2012.07.002. Epub 2012 Jul 11.
We report an analytical method using a thin film electro-acoustic resonator for the detection of organophosphorus pesticides. The acetylcholinesterase (AChE) enzyme was immobilized on the surface of the resonator. In the presence of organophosphorus compounds, the degree of inhibitory effect of organophosphorus compounds on the AChE activity and the concentration of pesticides were detected in real time by measuring the frequency shift of the resonator. The proposed device has a remarkably low detection limit of 1.8×10(-11)M and obvious advantages such as small size, simple operation, and integrated circuit compatibility, providing a promising tool for pesticide analysis.
我们报告了一种使用薄膜电声谐振器检测有机磷农药的分析方法。乙酰胆碱酯酶（AChE）酶被固定在谐振器的表面上。在存在有机磷化合物的情况下，通过测量谐振器的频率位移，实时检测有机磷化合物对 AChE 活性的抑制程度和农药的浓度。该装置具有非常低的检测限 1.8×10(-11)M 和明显的优势，如体积小、操作简单、与集成电路兼容，为农药分析提供了一种很有前途的工具。
