School of Materials Engineering, Purdue University, 701 West Stadium Avenue, West Lafayette, IN 47907, USA.
Anal Chim Acta. 2010 Feb 28;661(2):195-9. doi: 10.1016/j.aca.2009.12.020. Epub 2009 Dec 22.
Zinc oxide has been used as a matrix for immobilization of acetylcholinesterase (AChE) and detection of the pesticide paraoxon. The immobilized enzyme retained its enzymatic activity up to three months when stored in phosphate buffered saline (pH 7.4) at 4 degrees C. An amperometric biosensor for the detection of paraoxon was designed. The biosensor detected paraoxon in the range 0.035-1.38 ppm and can be used to detect other AChE inhibiting organophosphate pesticides.
氧化锌已被用作固定乙酰胆碱酯酶 (AChE) 和检测杀虫剂对氧磷的基质。当在磷酸盐缓冲盐水 (pH 7.4) 中于 4°C 储存时，固定化酶保持其酶活性长达三个月。设计了用于检测对氧磷的电流生物传感器。该生物传感器可检测 0.035-1.38ppm 范围内的对氧磷，可用于检测其他抑制乙酰胆碱酯酶的有机磷农药。
