Laboratoire de Virologie Est., Centre de Biologie et Pathologie Est., Hospices Civils de Lyon, Lyon, France.
Antiviral Res. 2012 Sep;95(3):224-8. doi: 10.1016/j.antiviral.2012.07.001. Epub 2012 Jul 14.
Discrimination between the mutations responsible for drug resistance and those of UL23 TK gene polymorphism can be difficult. A non-isotopic method has been developed to assess TK functionality by measuring monophosphate forms of both acyclovir (ACV) and thymidine using HPLC/DAD. Phenotypes of TKs could thus be characterized as TK altered (P84L, A189V, L227F), TK deficient (G200S, L291P) or TK partial (R163H). A reliable link between HSV UL23 TK mutations and ACV resistance is necessary for developing a powerful genotyping tool to detect ACV resistance quickly in clinical samples.
区分导致耐药性的突变和 UL23 TK 基因突变多态性可能具有一定难度。现已开发出一种非同位素方法，通过 HPLC/DAD 测量阿昔洛韦 (ACV) 和胸苷的单磷酸盐形式来评估 TK 功能。因此，可以将 TKs 表型描述为 TK 改变（P84L、A189V、L227F）、TK 缺乏（G200S、L291P）或 TK 部分改变（R163H）。为了开发一种强大的基因分型工具，在临床样本中快速检测 ACV 耐药性，需要建立 HSV UL23 TK 突变与 ACV 耐药性之间的可靠联系。
