Department of Chemistry, St. Joseph's College, Bangalore, Karnataka, India.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:91-6. doi: 10.1016/j.colsurfb.2012.06.020. Epub 2012 Jun 28.
An electrochemical sensor for sensitive detection of acetaminophen (AAP) was developed by electropolymerizing Patton and Reeder's reagent at carbon paste electrode (CPE). Modification improves the redox kinetics of AAP with increased current sensitivity. A similar modification at multiwall carbon nanotube (MWCNT) modified CPE did not result in an impressive charge transfer. Electrochemical impedance spectroscopy (EIS) of the bare and modified electrodes investigated imply a least charge transfer resistance at Patton and Reeder's reagent modified carbon paste electrode (MCPE/PR) as compared to bare CPE and MWCNT modified electrode. Differential pulse voltammetric (DPV) study at MCPE/PR electrode did not suffer any interference from its hydrolytic degradation product 4-aminophenol (4-AP) even in 1000-fold excess of its concentration and enables its detection simultaneously. A linear dynamic range of 0.7-100 μM with detection limit (S/N=3) of 0.53 μM was obtained for AAP. This modified electrode is easy to prepare, cheap, and having good reproducibility and stability. The analytical performance of the modified electrode is assessed by successfully applying it for the estimation of acetaminophen in different pharmaceutical samples and spiked biological fluid.
电化学传感器用于灵敏检测扑热息痛（AAP），通过在碳糊电极（CPE）上电聚合 Patton 和 Reeder 试剂来制备。修饰提高了 AAP 的氧化还原动力学，增加了电流灵敏度。在多壁碳纳米管（MWCNT）修饰的 CPE 上进行类似的修饰并没有导致令人印象深刻的电荷转移。对裸电极和修饰电极的电化学阻抗谱（EIS）研究表明，与裸 CPE 和 MWCNT 修饰电极相比，Patton 和 Reeder 试剂修饰的碳糊电极（MCPE/PR）的电荷转移电阻最小。在 MCPE/PR 电极上进行差分脉冲伏安法（DPV）研究时，即使在其水解降解产物 4-氨基酚（4-AP）浓度的 1000 倍过量的情况下，也不会受到任何干扰，并且可以同时对其进行检测。对于 AAP，获得了 0.7-100 μM 的线性动态范围和检测限（S/N=3）为 0.53 μM。该修饰电极制备简单、廉价，具有良好的重现性和稳定性。通过成功将其应用于不同药物样品和加标生物体液中扑热息痛的测定，评估了修饰电极的分析性能。
