Gavin B J, McMahon J A, McMahon A P
Roche Institute of Molecular Biology, Hoffmann-La Roche, Roche Research Center, Nutley, New Jersey 07110.
Genes Dev. 1990 Dec;4(12B):2319-32. doi: 10.1101/gad.4.12b.2319.
The mammary tumor-associated proto-oncogene Wnt-1/int-1 encodes a secreted protein implicated in the regulation of neural development in vertebrates and segmental pattern in Drosophila. Using a PCR-based strategy, we isolated cDNAs encoding six novel, related proteins that are expressed in fetal mice. Predicted proteins are of similar molecular masses (38-42 kD) and share between 50% and 85% of amino acids. All contain a putative hydrophobic signal sequence, and comparative analysis reveals 83 absolutely conserved amino acid residues, including 21 cysteines. Transcripts were detected throughout fetal development by Northern blot analysis. Detailed examination of the expression of two of these genes by in situ hybridization revealed complex temporal and spatial patterns of transcription. All new Wnt family members are expressed in adult tissues, particularly in brain and lung. These data support the view that the Wnt-1/int-1 family constitutes a large family of signaling peptides with diverse roles in mouse development.
乳腺肿瘤相关原癌基因Wnt-1/int-1编码一种分泌蛋白，该蛋白与脊椎动物神经发育调控及果蝇体节模式有关。利用基于聚合酶链反应（PCR）的策略，我们分离出了编码六种在胎鼠中表达的新型相关蛋白的互补脱氧核糖核酸（cDNA）。预测的蛋白质具有相似的分子量（38 - 42千道尔顿），氨基酸序列相似度在50%至85%之间。所有蛋白质都含有一个假定的疏水信号序列，比较分析显示有83个绝对保守的氨基酸残基，包括21个半胱氨酸。通过Northern印迹分析在整个胎儿发育过程中检测到了转录本。通过原位杂交对其中两个基因的表达进行详细检查，揭示了复杂的转录时空模式。所有新的Wnt家族成员都在成年组织中表达，特别是在脑和肺中。这些数据支持这样一种观点，即Wnt-1/int-1家族构成了一个在小鼠发育中具有多种作用的信号肽大家族。
