Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima 739-8527, Japan.
Water Sci Technol. 2012;66(5):958-64. doi: 10.2166/wst.2012.234.
Anaerobic ammonium oxidation (anammox) is a type of biological oxidation mediated by a group of Planctomycete-like bacteria. Members of the genus Candidatus Scalindua are mainly found in marine environments, but not exclusively. This group is cultured using different inoculums and conditions; however, its optimal growth conditions are not clear. Additionally, little information is known about the factors that influence the activity and the selection of a population of marine anammox bacteria. This study was conducted to investigate the influence of temperature and salinity on the marine anammox community. To accomplish this, an up-flow fixed-bed column reactor was operated, and quantitative fluorescence in situ hybridization (FISH) with probes specific to dominant marine anammox bacteria was conducted. Anammox activity was observed at 20 and 30 °C, but not at 10 °C. A nitrogen removal rate of 0.32 kg TN m(-3) day(-1) was obtained at 20 °C. These results suggest that temperature affects the activity (nitrogen removal rate) of anammox bacteria, while salinity does not affect the activity in the marine anammox biofilm.
厌氧氨氧化（anammox）是一类由类似浮霉菌的细菌介导的生物氧化过程。候选浮霉菌属（Candidatus Scalindua）的成员主要存在于海洋环境中，但并非仅限于此。该菌群可使用不同的接种物和条件进行培养；然而，其最佳生长条件尚不清楚。此外，对于影响海洋厌氧氨氧化菌种群活性和选择的因素知之甚少。本研究旨在探讨温度和盐度对海洋厌氧氨氧化群落的影响。为此，采用上流式固定床柱反应器进行操作，并使用针对优势海洋厌氧氨氧化菌的探针进行定量荧光原位杂交（FISH）。在 20 和 30°C 下观察到厌氧氨氧化活性，但在 10°C 下没有观察到。在 20°C 时，获得了 0.32 kg TN m(-3) day(-1) 的氮去除率。这些结果表明，温度会影响厌氧氨氧化菌的活性（氮去除率），而盐度不会影响海洋厌氧氨氧化生物膜中的活性。
