Service of Endocrinology, Diabetes and Metabolism, University Hospital CHUV, Lausanne, Switzerland.
Obes Facts. 2012;5(3):440-51. doi: 10.1159/000341137. Epub 2012 Jun 30.
To analyze the expression of peroxisome proliferator-activated receptor-γ1 and 2 (PPARγ1 and 2), 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), and leptin in adipose tissue (AT) of obese women during weight loss following Roux-en-Y gastric bypass (RYGB) and to compare these levels with those obtained in AT of nonobese subjects.
Gene expression was determined by real-time RT-PCR prior to surgery and at 3, 6, and 12 months after RYGB.
All obese patients lost weight, reaching a mean BMI of 29.3 ± 1.0 kg/m(2) at 1 year after surgery (-33.9 ± 1.5% of their initial body weight). In obese subjects leptin and 11βHSD1 were over-expressed, whereas PPARγ1 was expressed at lower levels compared to controls. After surgery, leptin and 11βHSD1 gene expression decreased, whereas PPARγ1 expression increased. At 12 months after RYGB, these 3 genes had reached levels similar to the controls. In contrast, PPARγ2 gene expression was not different between groups and types of tissue and remained unchanged during weight loss. We found a positive correlation between BMI and levels of gene expression of leptin and 11βHSD1.
Gene expression of leptin, PPARγ1, and 11βHSD1 in AT is modified in human obesity. This default is completely corrected by RYGB.
分析肥胖女性接受 Roux-en-Y 胃旁路术（RYGB）减肥前后脂肪组织（AT）中过氧化物酶体增殖物激活受体-γ1 和 2（PPARγ1 和 2）、11β-羟类固醇脱氢酶 1（11βHSD1）和瘦素的表达，并将这些水平与非肥胖受试者的 AT 中获得的水平进行比较。
在手术前和 RYGB 后 3、6 和 12 个月通过实时 RT-PCR 确定基因表达。
所有肥胖患者均减轻体重，术后 1 年体重指数（BMI）平均为 29.3 ± 1.0 kg/m²（比初始体重减轻 33.9 ± 1.5%）。肥胖患者中瘦素和 11βHSD1 过度表达，而 PPARγ1 的表达水平低于对照组。手术后，瘦素和 11βHSD1 的基因表达下降，而 PPARγ1 的表达增加。RYGB 后 12 个月，这 3 个基因的表达水平达到与对照组相似的水平。相比之下，PPARγ2 基因的表达在两组之间没有差异，且在体重减轻期间保持不变。我们发现 BMI 与瘦素和 11βHSD1 的基因表达水平呈正相关。
AT 中瘦素、PPARγ1 和 11βHSD1 的基因表达在人类肥胖中发生改变。RYGB 完全纠正了这种异常。
