儿童和成人脂肪组织中11β-羟类固醇脱氢酶1型和瘦素的特定储存库信使核糖核酸表达

Depot-specific messenger RNA expression of 11 beta-hydroxysteroid dehydrogenase type 1 and leptin in adipose tissue of children and adults.

作者信息

Li X, Lindquist S, Chen R, Myrnäs T, Angsten G, Olsson T, Hernell O

机构信息

Department of Clinical Sciences, Pediatrics, Umeå University, Umeå, Sweden.

出版信息

Int J Obes (Lond). 2007 May;31(5):820-8. doi: 10.1038/sj.ijo.0803470. Epub 2006 Oct 24.

DOI:10.1038/sj.ijo.0803470

PMID:17060929

Abstract

OBJECTIVE

To compare expression of messenger RNA (mRNA) coding for the cortisol regenerating enzyme 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1), and the adipocytokines leptin and resistin in paired biopsies of subcutaneous adipose tissue (SC) and omental adipose tissue (OM) from children.

DESIGN

Paired biopsies (SC and OM) were obtained from 54 children (age 0.17-16 years, body mass index (BMI) 12.5-28.3 kg/m(2), BMI standard deviation score (SDS) -2.5-4.5) and 16 adults (age 27-79 years, BMI 19-46 kg/m(2)) undergoing open abdominal surgery. mRNA levels of 11beta-HSD1, leptin and resistin were measured using quantitative real-time polymerase chain reaction (PCR).

RESULTS

11beta-HSD1 mRNA level was higher in OM than in SC (P<0.05), whereas leptin mRNA was higher in SC than in OM (P<0.001). There was no difference in the resistin mRNA level between SC and OM. These results were consistent in children and adults. In children, 11beta-HSD1 mRNA in SC was positively associated with BMI SDS (P<0.05), whereas in OM it was positively associated with age (P<0.05). The association between 11beta-HSD1 expression and age remained significant after adjustment for BMI SDS and gender. Leptin mRNA was positively associated with BMI SDS (SC: P<0.001, OM: P<0.001) but not with age in children. In multiple regression analyses, including anthropometric variables and age, BMI SDS was independently associated with mRNA levels of 11beta-HSD1 (P<0.05) and leptin (P<0.001) in SC. When normal weight and overweight children were analyzed separately, 11beta-HSD1 mRNA levels were positively associated with leptin in OM in the overweight group (P<0.05).

CONCLUSION

There are depot-specific differences in mRNA levels of 11beta-HSD1 and leptin in children and adults. The positive association of 11beta-HSD1 mRNA in OM with age may reflect a causal role in visceral fat accumulation during growth. Increasing 11beta-HSD1 and leptin mRNA in SC with increasing BMI SDS could suggest that the risk of metabolic consequences of obesity may be established early in life.

摘要

目的

比较儿童皮下脂肪组织（SC）和网膜脂肪组织（OM）配对活检样本中编码皮质醇再生酶11β-羟类固醇脱氢酶1型（11β-HSD1）的信使核糖核酸（mRNA）以及脂肪细胞因子瘦素和抵抗素的表达。

设计

从54名儿童（年龄0.17 - 16岁，体重指数（BMI）12.5 - 28.3 kg/m²，BMI标准差评分（SDS）-2.5 - 4.5）和16名接受开腹手术的成年人（年龄27 - 79岁，BMI 19 - 46 kg/m²）获取配对活检样本（SC和OM）。使用定量实时聚合酶链反应（PCR）测量11β-HSD1、瘦素和抵抗素的mRNA水平。

结果

OM中11β-HSD1 mRNA水平高于SC（P<0.05），而SC中瘦素mRNA水平高于OM（P<0.001）。SC和OM之间抵抗素mRNA水平无差异。这些结果在儿童和成年人中一致。在儿童中，SC中11β-HSD1 mRNA与BMI SDS呈正相关（P<0.05），而在OM中它与年龄呈正相关（P<0.05）。在调整BMI SDS和性别后，11β-HSD1表达与年龄之间的关联仍然显著。瘦素mRNA与BMI SDS呈正相关（SC：P<0.001，OM：P<0.001），但在儿童中与年龄无关。在包括人体测量变量和年龄的多元回归分析中，如果将正常体重和超重儿童分开分析，超重组中OM的11β-HSD1 mRNA水平与瘦素呈正相关（P<0.05）。