Radu Constantin, Adrar Hosna Sana, Alamir Ab, Hatherley Ian, Trinh Trung, Djaballah Hakim
Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
J Lab Autom. 2012 Oct;17(5):359-69. doi: 10.1177/2211068212453311. Epub 2012 Jul 12.
High-content screening (HCS) is becoming an accepted platform in academic and industry screening labs and does require slightly different logistics for execution. To automate our stand-alone HCS microscopes, namely, an alpha IN Cell Analyzer 3000 (INCA3000), originally a Praelux unit hooked to a Hudson Plate Crane with a maximum capacity of 50 plates per run, and the IN Cell Analyzer 2000 (INCA2000), in which up to 320 plates could be fed per run using the Thermo Fisher Scientific Orbitor, we opted for a 4 m linear track system harboring both microscopes, plate washer, bulk dispensers, and a high-capacity incubator allowing us to perform both live and fixed cell-based assays while accessing both microscopes on deck. Considerations in design were given to the integration of the alpha INCA3000, a new gripper concept to access the onboard nest, and peripheral locations on deck to ensure a self-reliant system capable of achieving higher throughput. The resulting system, referred to as Hestia, has been fully operational since the new year, has an onboard capacity of 504 plates, and harbors the only fully automated alpha INCA3000 unit in the world.
高内涵筛选（HCS）正在成为学术和工业筛选实验室中被广泛接受的平台，其执行所需的物流安排略有不同。为了实现我们的独立HCS显微镜自动化，即一台alpha IN Cell Analyzer 3000（INCA3000），它原本是一台Praelux设备，与一台Hudson Plate Crane相连，每次运行的最大容量为50个板，以及IN Cell Analyzer 2000（INCA2000），使用赛默飞世尔科技的Orbitor，每次运行最多可输送320个板，我们选择了一个4米长的线性轨道系统，该系统容纳两台显微镜、洗板机、大容量分配器和一个大容量培养箱，使我们能够在甲板上同时使用两台显微镜进行活细胞和固定细胞检测。在设计时考虑了alpha INCA3000的集成、一种用于访问机载巢的新夹具概念以及甲板上的周边位置，以确保系统能够自给自足并实现更高的通量。由此产生的系统被称为赫斯提亚（Hestia），自新年以来已全面投入使用，机载容量为504个板，并且是世界上唯一一台完全自动化的alpha INCA3000设备。
