Jensen Paul A, Dougherty Bonnie V, Moutinho Thomas J, Papin Jason A
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA.
J Lab Autom. 2015 Feb;20(1):51-5. doi: 10.1177/2211068214555414. Epub 2014 Nov 3.
We present a miniaturized plate reader for measuring optical density in 96-well plates. Our standalone reader fits in most incubators, environmental chambers, or biological containment suites, allowing users to leverage their existing laboratory infrastructure. The device contains no moving parts, allowing an entire 96-well plate to be read several times per second. We demonstrate how the fast sampling rate allows our reader to detect small changes in optical density, even when the device is placed in a shaking incubator. A wireless communication module allows remote monitoring of multiple devices in real time. These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.
我们展示了一种用于测量96孔板光密度的小型化酶标仪。我们的独立式酶标仪可适配大多数培养箱、环境试验箱或生物安全柜，使用户能够利用其现有的实验室基础设施。该设备没有移动部件，每秒可对整个96孔板进行多次读数。我们展示了即使将该设备置于振荡培养箱中，快速采样率也能使我们的酶标仪检测到光密度的微小变化。无线通信模块允许对多个设备进行实时远程监控。这些特性便于组装多个酶标仪，从而为高通量表型筛选创建一个可扩展、准确的解决方案。
