Centre for Research in Ceramic and Composite Materials (CICECO), Department of Ceramics and Glass Engineering, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
J Appl Biomater Funct Mater. 2012 Sep 27;10(2):92-8. doi: 10.5301/JABFM.2012.9254.
The formation of a calcium phosphate layer on the surface of bone tissue engineering biomaterials is crucial for their integration in bone. Simulated biological fluids used to study the in vitro formation of those layers do not usually contain important organic components present in vivo-notably proteins. In this work the influence of bovine serum albumin on the mineralization process of poly(methylmethacrylate)-based composite was studied in vitro.
The effect of protein on calcium phosphate formation was followed by ion concentration analyses (ICP), x-ray diffraction (XRD), and scanning electron microscopy coupled with x-ray energy dispersive spectroscopy (SEM-EDS).
The results showed the precipitation of a calcium phosphate layer on the surface of composites immersed in SBF and SBFA. Further transformation and crystallization of this layer, initially amorphous, appears to be influenced by the presence of albumin.
在骨组织工程生物材料表面形成磷酸钙层对于其与骨的整合至关重要。用于研究这些层体外形成的模拟生物液通常不含体内存在的重要有机成分，特别是蛋白质。在这项工作中，研究了牛血清白蛋白对基于聚甲基丙烯酸甲酯的复合材料的矿化过程的影响。
通过离子浓度分析（ICP）、X 射线衍射（XRD）和扫描电子显微镜结合 X 射线能量色散光谱（SEM-EDS）来研究蛋白质对钙磷形成的影响。
结果表明，在 SBF 和 SBFA 中浸泡的复合材料表面沉淀出一层磷酸钙。最初是非晶态的这种层的进一步转化和结晶似乎受到白蛋白的存在的影响。
