Gheorghe Asachi Technical University, Faculty of Chemical Engineering and Environmental Protection, Department of Chemical Engineering, Iasi, Romania; Grigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, Iasi, Romania.
Grigore T. Popa University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Sciences, Iasi, Romania.
Int J Biol Macromol. 2019 Mar 15;125:612-620. doi: 10.1016/j.ijbiomac.2018.12.083. Epub 2018 Dec 8.
Composites based on combination of biopolymers (chitosan, hyaluronic acid and bovine serum albumin or gelatin), calcium phosphates (CP) and magnetic nanoparticles have been prepared by a biomimetic co-precipitation method. The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. The morphology of the magnetic composites, studied using scanning electron microscopy (SEM) indicated a macroporous structure, which influenced the retention of simulated biological fluids. Fourier transformed infrared spectroscopy and X-ray diffraction and Energy-dispersive X-ray spectroscopy (EDX) confirmed the composition of the scaffolds and the formation of various types of calcium phosphates with amorphous nature. The in vitro degradation studies showed a slow degradation process for magnetic composites that confirmed the tightly connection of the polymeric matrix with calcium phosphates, which limits the enzyme access to the degradable components and material disintegration. The magnetic scaffolds exhibited no negative effect on osteoblasts cell, emphasizing a good biocompatibility. Considering the scaffolds properties, some compositions based on calcium phosphates, chitosan, Hya/Bsa and more than 3% of MNPs are recommended for further optimization and in vivo tests.
基于生物聚合物（壳聚糖、透明质酸和牛血清白蛋白或明胶）、磷酸钙（CP）和磁性纳米颗粒的复合材料已通过仿生共沉淀方法制备。仿生策略受到天然矿化过程的启发，在生理温度和 pH 条件下，合成的矿物质通常与蛋白质、多糖或其他矿物质形式结合形成复合材料。使用扫描电子显微镜（SEM）研究的磁性复合材料的形态表明具有大孔结构，这影响了模拟生物流体的保留。傅里叶变换红外光谱、X 射线衍射和能量色散 X 射线光谱（EDX）证实了支架的组成以及各种类型的具有非晶态特性的磷酸钙的形成。体外降解研究表明磁性复合材料具有缓慢的降解过程，这证实了聚合物基质与磷酸钙的紧密连接，限制了酶对可降解成分的作用和材料的崩解。磁性支架对成骨细胞没有负面影响，强调了良好的生物相容性。考虑到支架的特性，建议对基于磷酸钙、壳聚糖、Hya/Bsa 和超过 3%的 MNPs 的一些成分进行进一步优化和体内测试。
