Department of Health Sciences, Università del Piemonte Orientale Amedeo Avogadro, Novara, Italy.
J Appl Biomater Funct Mater. 2012 Sep 27;10(2):99-106. doi: 10.5301/JABFM.2012.9257.
Natural resources are receiving growing interest because of their possible conversion from a cheap and easily available material into a biomedical product. Cuttlefish bone from Sepia Officinalis was investigated in order to obtain an hydroxyapatite porous scaffold using hydrothermal transformation.
Complete conversion of the previous calcium carbonate (aragonite) phase into a calcium phosphate (hydroxyapatite) phase was performed with an hydrothermal transformation at 200 °C (~ 15 atm), for four hours, with an aqueous solution of KH2PO4 in order to set the molar ratio Ca/P = 10/6 in a reactor (Parr 4382). The complete conversion was then analyzed by TGA, ATR-FTIR, x-ray diffraction, and SEM. Moreover, the material was biologically investigated with MC3T3-E1 in static cultures, using both osteogenic and maintenance media. The expression of osteogenic markers as ALP and osteocalcin and the cell proliferation were investigated.
Cuttlefish bone has been successfully transformed from calcium carbonate into calcium phosphate. Biological characterization revealed that osteogenic markers are expressed using both osteogenic and maintenance conditions. Cell proliferation is influenced by the static culture condition used for this three-dimensional scaffold.
The new scaffold composed by hydroxyapatite and derived for a natural source presents good biocompatibility and can be used for further investigations using dynamic cultures in order to improve cell proliferation and differentiation for bone tissue engineering.
由于自然资源可能从廉价且易得的材料转化为生物医学产品，因此它们越来越受到关注。本研究旨在利用水热转化法从乌贼骨中提取羟磷灰石多孔支架。
通过在 200°C（约 15 个大气压）下的水热转化，在反应器（Parr 4382）中使用 KH2PO4 的水溶液将前碳酸钙（霰石）相完全转化为磷酸钙（羟磷灰石）相，以实现摩尔比 Ca/P = 10/6。通过 TGA、ATR-FTIR、X 射线衍射和 SEM 分析完全转化。此外，通过静态培养，使用成骨和维持培养基，在 MC3T3-E1 中对该材料进行了生物学研究。研究了碱性磷酸酶和骨钙素等成骨标志物的表达和细胞增殖。
乌贼骨已成功地从碳酸钙转化为磷酸钙。生物学特性表明，使用成骨和维持条件均可表达成骨标志物。细胞增殖受用于这种三维支架的静态培养条件的影响。
由天然来源的羟磷灰石组成的新型支架具有良好的生物相容性，可用于进一步的动态培养研究，以改善细胞增殖和分化，用于骨组织工程。
