Xin Hongmei, Guo Zhengyan, Fan Lixia, Hu Zhaonong, Wu Wenjun
Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
Wei Sheng Wu Xue Bao. 2012 Apr 4;52(4):435-41.
In order to study diversity and find antimicrobial activities of actinomycetes from pesticide-contaminated spots in Shandong Peninsula.
The phylogenetic analysis of 154 isolated strains was done based on 16SrDNA sequences. Antimicrobial activities of 10 non-Streptomyces strains were tested by using cylinder-plate method and hypha growth rate method.
Among the strains, 154 strains belonged to 7 families, 8 genera: Streptomyces (87.01%), Kocuria, Microbacterium, Nocardiopsis, Knoellia, Pseudonocardia, Micromonospora, Actinoplanes. The fermentation broths of 10 non-Streptomyces strains had inhibitory actives against all tested phytopathogenic fungi (Botrytis cinerea, Fusarium oxysporum f. sp. niveum, Gibberella zeae, Sclerotinia sclerotiorum, Colletotrichum gloesporioides) and bacteria (Staphylococcus aureus, Bacillus subtills, Bacillus cereus, Escherichai coli, Pseudomonas aeruginos) , especially Microbacterium oxydans JN853773 and Kocuria rosea JN192402 had strong inhibitory effects.
Abundant diversity of actinomycetes existed in pesticide contaminated spots in Shandong Peninsula. Microbacterium oxydans JN853773 and Kocuria rosea JN192402 showed high antimicrobial activities and could be further exploited.
研究山东半岛农药污染点放线菌的多样性并寻找其抗菌活性。
基于16SrDNA序列对154株分离菌株进行系统发育分析。采用滤纸片法和菌丝生长速率法检测10株非链霉菌菌株的抗菌活性。
这些菌株分属于7科8属，其中链霉菌属（87.01%）、考克氏菌属、微杆菌属、诺卡氏放线菌属、克内氏菌属、假诺卡氏菌属、小单孢菌属、游动放线菌属。10株非链霉菌菌株的发酵液对所有供试植物病原菌（灰葡萄孢、西瓜枯萎病菌、禾谷镰刀菌、核盘菌、胶孢炭疽菌）和细菌（金黄色葡萄球菌、枯草芽孢杆菌、蜡样芽孢杆菌、大肠杆菌、铜绿假单胞菌）均有抑制活性，尤其是氧化微杆菌JN853773和玫瑰考克氏菌JN192402具有较强的抑制作用。
山东半岛农药污染点存在丰富的放线菌多样性。氧化微杆菌JN853773和玫瑰考克氏菌JN192402表现出较高的抗菌活性，可进一步开发利用。
