Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), P. O. Box, 31535-1897, Karaj, Iran.
Department of Food Science and Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.
Sci Rep. 2022 Jan 10;12(1):405. doi: 10.1038/s41598-021-04103-z.
The carbohydrate-hydrolyzing enzymes play a crucial role in increasing the phenolic content and nutritional properties of polysaccharides substrate, essential for cost-effective industrial applications. Also, improving the feed efficiency of poultry is essential to achieve significant economic benefits. The current study introduced a novel thermostable metagenome-derived xylanase named PersiXyn8 and investigated its synergistic effect with previously reported α-amylase (PersiAmy3) to enhance poultry feed utilization. The potential of the enzyme cocktail in the degradation of poultry feed was analyzed and showed 346.73 mg/g poultry feed reducing sugar after 72 h of hydrolysis. Next, the impact of solid-state fermentation on corn quality was investigated in the presence and absence of enzymes. The phenolic content increased from 36.60 mg/g GAE in control sample to 68.23 mg/g in the presence of enzymes. In addition, the enzyme-treated sample showed the highest reducing power OD 700 of 0.217 and the most potent radical scavenging activity against ABTS (40.36%) and DPPH (45.21%) radicals. Moreover, the protein and ash contents of the fermented corn increased by 4.88% and 6.46%, respectively. These results confirmed the potential of the carbohydrate-hydrolyzing enzymes cocktail as a low-cost treatment for improving the phenolic content, antioxidant activity, and nutritional values of corn for supplementation of corn-based poultry feed.
碳水化合物水解酶在提高多糖底物的酚含量和营养价值方面发挥着关键作用，这对于具有成本效益的工业应用至关重要。此外，提高家禽的饲料效率对于实现显著的经济效益至关重要。本研究介绍了一种新型耐热的宏基因组来源的木聚糖酶，名为 PersiXyn8，并研究了其与先前报道的α-淀粉酶（PersiAmy3）的协同作用，以提高家禽饲料的利用率。分析了酶混合物在降解家禽饲料方面的潜力，水解 72 小时后，产生了 346.73 mg/g 家禽饲料还原糖。接下来，研究了在存在和不存在酶的情况下，固态发酵对玉米质量的影响。酚含量从对照样品中的 36.60 mg/g GAE 增加到酶存在时的 68.23 mg/g。此外，酶处理的样品的 OD700 还原能力最高，达到 0.217，对 ABTS（40.36%）和 DPPH（45.21%）自由基的清除能力最强。此外，发酵玉米的蛋白质和灰分含量分别增加了 4.88%和 6.46%。这些结果证实了碳水化合物水解酶混合物作为一种低成本处理方法的潜力，可提高玉米的酚含量、抗氧化活性和营养价值，以补充基于玉米的家禽饲料。
