Department of Sensitive Organs, Policlinic Umberto I, Sapienza University of Rome, Rome, Italy.
Connect Tissue Res. 2013;54(1):1-4. doi: 10.3109/03008207.2012.712584.
Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC β1, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, and PI-PLC ϵ were expressed. PI-PLC β1 was weakly expressed, PI-PLC δ4 was inconstantly expressed, and PI-PLC γ2 was weakly expressed.
成纤维细胞参与许多受不同信号转导途径调控的功能，包括磷酸肌醇（PI）信号系统和相关的转化酶，如磷酸肌醇特异性磷脂酶 C（PI-PLC）。PI-PLC 家族包含 PI 信号转导途径中的关键效应酶。一旦被激活，PI-PLC 就会将重要的膜 PI，即磷脂酰肌醇（4,5）二磷酸，切割成肌醇三磷酸和二酰基甘油——这两者都是信号转导中的关键分子。据报道，在成纤维细胞中存在某些 PI-PLC 酶的活性，尽管完整的表达谱尚不清楚。每种细胞类型都表达一组选定的 PI-PLC 同工型，而表达谱的知识是进一步研究的必要和初步工具。在本研究中，我们描绘了人皮肤成纤维细胞中 PI-PLC 酶的表达谱。表达了 PI-PLCβ1、PI-PLCβ3、PI-PLCβ4、PI-PLCγ1、PI-PLCγ2、PI-PLCδ1、PI-PLCδ3、PI-PLCδ4 和 PI-PLCε。PI-PLCβ1 表达较弱，PI-PLCδ4 表达不稳定，PI-PLCγ2 表达较弱。
