磷酸肌醇特异性磷脂酶 C 酶在正常子宫内膜和子宫内膜异位症中的表达。
Expression of phosphoinositide-specific phospholipase C enzymes in normal endometrium and in endometriosis.
机构信息
Department of Sensitive Organs, Policlinic Umberto I, Sapienza University, Rome, Italy.
出版信息
Fertil Steril. 2012 Aug;98(2):410-4. doi: 10.1016/j.fertnstert.2012.04.020. Epub 2012 May 17.
OBJECTIVE
To delineate the panel of expression of phosphoinositide-specific phospholipase C (PI-PLC) signaling enzymes in normal endometrium and in endometriosis.
DESIGN
Clinical/experimental study.
SETTING
University.
PATIENT(S): Healthy donor woman and endometriosis-affected woman.
INTERVENTION(S): Normal endometrium and endometriosis surgical biopsies were analyzed using gene expression analyses methodology (reverse transcriptase-polymerase chain reaction [PCR], bioanalyses).
MAIN OUTCOME MEASURE(S): Gene expression (messenger RNA concentration) measures of 12 PI-PLC enzymes: PI-PLC β1, PI-PLC β2, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, PI-PLC ε, PI-PLC η1, and PI-PLC η2.
RESULT(S): PI-PLC β1, PI-PLC β3, PI-PLC δ1, and PI-PLC δ3 enzymes were detected, although differently expressed in normal and endometriosis tissues.
CONCLUSION(S): The involvement of PI-PLC enzymes in inflammation and the consistency of susceptible endometriosis loci with PI-PLC genes mapping corroborate the hypothesis that PI signaling might be involved in the pathogenesis of endometriosis.
目的
描绘磷酸肌醇特异性磷脂酶 C（PI-PLC）信号酶在正常子宫内膜和子宫内膜异位症中的表达谱。
设计
临床/实验研究。
地点
大学。
患者
健康供体女性和子宫内膜异位症患者。
干预措施
采用基因表达分析方法（逆转录-聚合酶链反应[PCR]、生物分析）分析正常子宫内膜和子宫内膜异位症手术活检。
主要观察指标
12 种 PI-PLC 酶的基因表达（信使 RNA 浓度）测量值：PI-PLCβ1、PI-PLCβ2、PI-PLCβ3、PI-PLCβ4、PI-PLCγ1、PI-PLCγ2、PI-PLCδ1、PI-PLCδ3、PI-PLCδ4、PI-PLCε、PI-PLCη1 和 PI-PLCη2。
结果
检测到 PI-PLCβ1、PI-PLCβ3、PI-PLCδ1 和 PI-PLCδ3 酶，但在正常和子宫内膜异位症组织中的表达不同。
结论
PI-PLC 酶参与炎症，易感子宫内膜异位症基因座与 PI-PLC 基因图谱的一致性，支持 PI 信号可能参与子宫内膜异位症发病机制的假说。
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