使用光子计数成像仪原位检测微孔板上的放射性。

Detection of radioactivity on microtiterplates in situ using a photon-counting imager.

作者信息

Joss U R, Schleinkofer L

机构信息

CIBA-GEIGY Ltd., Research Department Pharmaceutical Division, Basel, Switzerland.

出版信息

J Biochem Biophys Methods. 1990 Sep-Oct;21(3):217-26. doi: 10.1016/0165-022x(90)90015-5.

DOI:10.1016/0165-022x(90)90015-5

PMID:2280055

Abstract

A position-sensitive single-photon counting imaging system, which can determine intensity and location of a light source, has been used for the detection of 125I-labeled Interleukin-1 and [3H]thymidine in 96 wells of a microtiter plate (MTP) simultaneously. 4 Bq (1 Bq = 1 Bequerel = 1 disintegration/s), 22 and 150 Bq of 125I and 3, 10 and 100 Bq of 3H were visualized and quantified by transforming the radioactivity into light in the visible range by means of Xtalscint, a solid scintillator. After only 1 min of photon accumulation time, the highest radioactivity of both isotopes could be clearly distinguished from background. Photon counts correlate well with radioactivity measured in a beta-counter (for 3H) and a gamma-counter (for 125I). The overall counting efficiency was about 5% for 125I and 3% for 3H.

摘要

一种位置敏感型单光子计数成像系统，可确定光源的强度和位置，已被用于同时检测微量滴定板（MTP）96孔中的125I标记白细胞介素-1和[3H]胸腺嘧啶核苷。借助固体闪烁体Xtalscint将放射性转化为可见光范围内的光，从而对4 Bq（1 Bq = 1贝可勒尔 = 1次衰变/秒）、22 Bq和150 Bq的125I以及3 Bq、10 Bq和100 Bq的3H进行可视化和定量分析。仅经过1分钟的光子积累时间，两种同位素的最高放射性就能与背景清晰区分。光子计数与在β计数器（用于3H）和γ计数器（用于125I）中测得的放射性具有良好的相关性。125I的总体计数效率约为5%，3H的总体计数效率约为3%。