[Nav1.8在人牙髓中的表达]

[Expression of Nav1.8 in human dental pulp].

作者信息

Tao Ran, Jiang Yong

机构信息

Department of Preventive Dentistry, School of Stomatology, Anhui Medical University, Hefei 230032, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2012 Mar;47(3):177-81. doi: 10.3760/cma.j.issn.1002-0098.2012.03.012.

DOI:10.3760/cma.j.issn.1002-0098.2012.03.012

PMID:22800673

Abstract

OBJECTIVE

To investigate the relationship between dental pain and Nav1.8 expression level by detecting the expression of voltage-gated sodium channel Nav1.8 in normal human dental pulps and painful pulp tissues.

METHODS

Immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of Nav1.8 in normal human dental pulp and painful dental pulp.

RESULTS

Nav1.8 expressed in dental pulps and the expression level of Nav1.8 increased significantly in painful dental pulps in comparison with normal dental tissues. The immunohistochemistry results revealed that Nav1.8 expression level in painful dental issue was 0.547 ± 0.049 in relative intensity,and in normal dental issue 0.356 ± 0.058 (P < 0.05). Western blotting showed similar results of 0.234 ± 0.030 vs 0.108 ± 0.012. RT-PCR results indicated that Nav1.8 mRNA expression level in painful dental issue was 7.130 ± 2.471 and in normal dental issue was 1.024 ± 0.295 (P < 0.05).

CONCLUSIONS

The expression level of Nav1.8 increased significantly in painful dental pulp tissue, suggesting that Nav1.8 may play an important role in the development and transmission of dental pain.

摘要

目的

通过检测正常人牙髓组织和疼痛牙髓组织中电压门控钠通道Nav1.8的表达，探讨牙痛与Nav1.8表达水平之间的关系。

方法

采用免疫组织化学、逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法检测正常人牙髓和疼痛牙髓中Nav1.8的表达。

结果

Nav1.8在牙髓中表达，与正常牙髓组织相比，疼痛牙髓中Nav1.8的表达水平显著升高。免疫组织化学结果显示，疼痛牙髓组织中Nav1.8表达水平的相对强度为0.547±0.049，正常牙髓组织中为0.356±0.058（P<0.05）。蛋白质印迹法显示了相似的结果，分别为0.234±0.030和0.108±0.012。RT-PCR结果表明，疼痛牙髓组织中Nav1.8 mRNA表达水平为7.130±2.471，正常牙髓组织中为1.024±0.295（P<0.05）。