Division of Pediatric Dentistry, Department of Orthodontics and Pediatric Dentistry, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Int Endod J. 2012 Apr;45(4):372-8. doi: 10.1111/j.1365-2591.2011.01986.x. Epub 2011 Nov 16.
To investigate alterations in Na(V) 1.8 and Na(V) 1.9 expression within inflamed dental pulp tissue of human primary teeth.
Dental pulp tissue obtained from both normal and inflamed pulps in primary teeth as well as pulps from normal and inflamed permanent teeth was used. The quantity of Na(V) 1.8 and Na(V) 1.9 expression in the dental pulp tissue was investigated using Western blot analysis. General neuron marker (PGP9.5) was used to quantify for neural density, and an increase in metalloproteinase-9 was used to indicate pulpal inflammation in inflamed teeth. Statistically significant differences for each determined parameter between normal and inflamed teeth of both primary and permanent teeth were tested using the Mann-Whitney rank sum test.
There was no significant difference in neural density of normal and inflamed dental pulp tissue, although degrees of inflammation were increased in the inflamed dental pulp of both permanent and primary teeth (P < 0.05). Na(V) 1.8 and Na(V) 1.9 expression in inflamed pulps of permanent teeth increased significantly compared with normal permanent teeth (P < 0.05). However, only Na(V) 1.8 expression was increased significantly in the inflamed dental pulp of primary teeth (P < 0.05).
Na(V) 1.8 alone may be the therapeutic target for treatment of painful pulpitis in primary teeth.
研究人原发性牙髓组织炎症时 Na(V) 1.8 和 Na(V) 1.9 表达的变化。
使用来自原发性正常和炎症牙髓以及来自正常和炎症恒牙髓的牙髓组织。采用 Western blot 分析检测牙髓组织中 Na(V) 1.8 和 Na(V) 1.9 的表达量。用一般神经元标志物（PGP9.5）来定量神经密度，用金属蛋白酶-9 的增加来表示炎症牙髓中牙齿的炎症。使用 Mann-Whitney 秩和检验测试原发性和恒性牙齿正常和炎症牙齿之间每个确定参数的统计学差异。
尽管恒牙髓的炎症程度增加（P < 0.05），但正常和炎症牙髓的神经密度没有显著差异。与正常恒牙髓相比，恒牙髓炎症时 Na(V) 1.8 和 Na(V) 1.9 的表达显著增加（P < 0.05）。然而，只有原发性牙髓炎症时 Na(V) 1.8 的表达显著增加（P < 0.05）。
Na(V) 1.8 可能是治疗原发性牙髓痛性炎症的治疗靶点。
