隐睾小鼠治疗后凋亡基因表达及其蛋白的评估。

Evaluation of apoptotic genes expression and its protein after treatment of cryptorchid mice.

作者信息

Absalan Forouzan, Movahedin Mansoureh, Mowla Seyed Javad

机构信息

Dept. of Anatomical Sciences, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.

Dept. of Anatomy, Medical Faculty, Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Iran Biomed J. 2012;16(2):77-83. doi: 10.6091/ibj.1035.2012.

DOI:10.6091/ibj.1035.2012

PMID:22801280

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3600956/

Abstract

INTRODUCTION

Cryptorchidism has been proved to cause apoptosis in germ cells in respond to changes in the stimulation levels of specific physiological events. The purpose of this study was to determine whether treatment of bilateral cryptorchidism was associated with alterations in testicular gene expression.

METHODS

To induce bilateral cryptorchid model, immature mice were anesthetized and a small incision was made in the abdominal skin and peritoneum, then fat pad at the upper end of testis was sutured to the peritoneum. Transcript level of Bax, Bcl-2 proper, p53 and survivin mRNA and protein were determined after performing the two treatment methods: surgical return of testis into scrotum (Exp1) and transplantation of spermatogonial stem cells with later orchidopexy (Exp2), performed 2 and 3 months after heat exposure, respectively.

RESULTS

RT-PCR data showed decreased levels of p53 and Bax expression as well as decreased levels of Bcl-2 mRNA in treatment groups especially after transplantation compared with control group. The expression of survivin 140 was increased significantly after treatment, whereas that of survivin 40 was lower especially in the orchidopexy group. Immunohistochemistry staining showed that the intensity of Bax expression mainly was decreased in treated cryptorchid testis and rates of Bcl-2 were increased significantly, but expression of p53 and survivin proteins did not changed significantly after treatment.

DISCUSSION

These observations suggest that cell-type-specific and many apoptotic systems control germ cell apoptosis after treatment of cryptorchidism.

摘要

引言

隐睾症已被证明会因特定生理事件刺激水平的变化而导致生殖细胞凋亡。本研究的目的是确定双侧隐睾症的治疗是否与睾丸基因表达的改变有关。

方法

为诱导双侧隐睾模型，将未成熟小鼠麻醉，在腹部皮肤和腹膜上做一个小切口，然后将睾丸上端的脂肪垫缝合到腹膜上。在分别于热暴露后2个月和3个月进行两种治疗方法后，即手术将睾丸放回阴囊（实验1）和精原干细胞移植后进行睾丸固定术（实验2），测定Bax、Bcl-2、p53和survivin mRNA及蛋白的转录水平。

结果

逆转录聚合酶链反应（RT-PCR）数据显示，与对照组相比，治疗组尤其是移植后，p53和Bax表达水平降低，Bcl-2 mRNA水平也降低。治疗后survivin 140的表达显著增加，而survivin 40的表达较低，尤其是在睾丸固定术组。免疫组织化学染色显示，治疗后的隐睾睾丸中Bax表达强度主要降低，Bcl-2的表达率显著增加，但治疗后p53和survivin蛋白的表达没有显著变化。