EPO 与肾小球系膜细胞中 FN 表达的相关性。
The association between RhEPO and FN expression in glomerular mesangial cells.
机构信息
Emergency Department and Surgical Intensive Care Unit, Shanghai 10th People's Hospital Tongji University School of Medicine. Shanghai, China.
出版信息
Panminerva Med. 2012 Sep;54(3):205-9.
AIM
Erythropoietin (rhEPO) is increasingly being used in the treatment of anemia caused by miscellaneous reasons. The aim of our research is to investigate the effects of recombinant human erythropoietin (rhEPO) on cellular growth and fibronectin (FN) expression in glomerular mesangial cells.
METHODS
Western blot was used to detect the expression of FN induced by rhEPO (1, 10, 100 and 1000U/mL). In vivo studies, male CD-1 mice were administered rhEPO subcutaneously at a single dose of 1000U/Kg. The cellular hypertrophy was quantified by counting cell number and calculating the ratio of cell protein to cell number.
RESULTS
- Compared with the control group, the results of mesangial cells' growth stimulated by rhEPO were not significantly different; 2) RhEPO lead to hypertrophy of mesangial cells; 3) rhEPO induced FN expression of mesangial cell in a dose-dependent way. Compared to control, 100U/mL rhEPO enhanced the expression of FN significantly; 4) indirect immunofluorescence showed that rhEPO induced large amount deposition of FN in the intercellular space of mesangial cells; 5) in vivo studies, there were markedly increase of FN expression in mice received the injection of rhEPO.
CONCLUSION
RhEPO could up-regulated the expression of FN and induced glomerular mesangial cells hypertrophy. These results suggested rhEPO could induce dysfunction of renal glomerulus through its influence on the function of mesangial cells.
目的
促红细胞生成素（rhEPO）越来越多地被用于治疗各种原因引起的贫血。我们的研究目的是探讨重组人促红细胞生成素（rhEPO）对肾小球系膜细胞细胞生长和纤维连接蛋白（FN）表达的影响。
方法
采用 Western blot 检测 rhEPO（1、10、100 和 1000U/ml）诱导的 FN 表达。体内研究中，雄性 CD-1 小鼠单次皮下给予 rhEPO 1000U/kg。通过计数细胞数并计算细胞蛋白与细胞数的比值来定量细胞肥大。
结果
1）与对照组相比，rhEPO 刺激系膜细胞生长的结果无显著差异；2）rhEPO 导致系膜细胞肥大；3）rhEPO 以剂量依赖的方式诱导系膜细胞 FN 表达。与对照组相比，100U/ml rhEPO 显著增强 FN 的表达；4）间接免疫荧光显示 rhEPO 诱导大量 FN 沉积在系膜细胞的细胞间隙；5）体内研究表明，接受 rhEPO 注射的小鼠 FN 表达明显增加。
结论
rhEPO 可上调 FN 的表达并诱导肾小球系膜细胞肥大。这些结果表明，rhEPO 通过对系膜细胞功能的影响，可导致肾小球功能障碍。
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