Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
Poult Sci. 2012 Aug;91(8):1833-7. doi: 10.3382/ps.2012-02202.
Thymic CD4+CD25+ cells from turkeys were analyzed for T regulatory cell suppressive properties and cytokine production profile. Thymocytes treated with concanavalin-A and interleukin-2 upregulated CD25 at 72 h of culture. The mean CD4+CD25+ cell percentage in the blood was 1.1%, in the thymus was 2.7%, in the spleen was 9.6%, in the lung was 18.6%, in cecal tonsils was 9.2%, and in bone marrow was 14.9%. The CD4+CD25+ cells were not detectable in the bursa. Turkey thymic CD4+CD25+ cells had approximately 158-fold higher interleukin-10 mRNA, 7-fold higher transforming growth factor β, 24-fold higher cytotoxic T lymphocyte antigen 4, and 11-fold higher lymphocyte-activation gene 3 mRNA amounts than thymic CD4+CD25- cells. Thymic CD4+CD25+ cells had no detectable levels of interleukin-2 mRNA. Turkey CD4+CD25+ cells suppressed naïve cell proliferation in vitro. It could be concluded that thymic CD4+CD25+ cells in turkeys are likely the counterpart of mammalian T regulatory cells.
从火鸡的胸腺中分析 CD4+CD25+细胞的 T 调节细胞抑制特性和细胞因子产生谱。用刀豆蛋白 A 和白细胞介素-2 处理的胸腺细胞在培养的 72 小时上调 CD25。血液中 CD4+CD25+细胞的平均百分比为 1.1%，胸腺为 2.7%，脾脏为 9.6%，肺脏为 18.6%，盲肠扁桃体为 9.2%，骨髓为 14.9%。B ursa 中未检测到 CD4+CD25+细胞。火鸡胸腺 CD4+CD25+细胞的白细胞介素-10 mRNA 约高 158 倍，转化生长因子-β高 7 倍，细胞毒性 T 淋巴细胞抗原 4 高 24 倍，淋巴细胞激活基因 3 mRNA 高 11 倍，而胸腺 CD4+CD25-细胞则没有检测到白细胞介素-2 mRNA。火鸡 CD4+CD25+细胞在体外抑制幼稚细胞增殖。可以得出结论，火鸡的胸腺 CD4+CD25+细胞可能是哺乳动物 T 调节细胞的对应物。
