Pós-Graduação em Doenças Infecciosas e Parasitárias, Faculdade de Medicina e Departamento de Patologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, Brazil.
Am J Trop Med Hyg. 2012 Sep;87(3):470-2. doi: 10.4269/ajtmh.2012.11-0534. Epub 2012 Jul 16.
We investigated the occurrence of Leishmania infantum chagasi in Didelphis albiventris opossums at a wild animal rehabilitation center in the city of Campo Grande, Brazil. A total of 54 opossums were tested for L. i. chagasi infection in peripheral blood and bone marrow samples. The samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction-restriction fragment length polymorphism. Leishmania i. chagasi DNA was detected by polymerase chain reaction-restriction fragment length polymorphism in 11 (20.37%) animals. A total of 81.81% of positive opossums were captured in areas of known visceral leishmaniasis transmission. These results suggest a role for D. albiventris in the urban transmission of visceral leishmaniasis.
我们调查了在巴西坎波格兰德市的一个野生动物康复中心,白足负鼠中查加斯利什曼原虫的发生情况。共有 54 只负鼠的外周血和骨髓样本进行了利什曼原虫感染检测。通过直接检查、在特定培养基中培养和聚合酶链反应-限制性片段长度多态性分析来分析样本。聚合酶链反应-限制性片段长度多态性检测到 11 只(20.37%)动物携带查加斯利什曼原虫 DNA。阳性负鼠中有 81.81%是在已知内脏利什曼病传播区域捕获的。这些结果表明白足负鼠在城市内脏利什曼病传播中起作用。