Laboratório de Sistemática Bioquímica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Rev Soc Bras Med Trop. 2014 Mar-Apr;47(2):243-6. doi: 10.1590/0037-8682-0007-2013.
INTRODUCTION: During a diagnostic evaluation of canine visceral leishmaniasis (VL), two of seventeen dogs were found to be co-infected by Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi. METHODS: Specific polymerase chain reaction (PCR) and restriction fragment length polymorphism-PCR (RFLP-PCR) assays were performed. RESULTS: PCR assays for Leishmania subgenus identification followed by RFLP-PCR analysis in biopsies from cutaneous lesions and the spleen confirmed the presence of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in those fragments. CONCLUSIONS: This report reinforces the importance of using serological and molecular techniques in the epidemiological surveillance of canine populations in endemic areas in which both diseases are known to co-exist. In such cases, a reassessment of the control measures is required.
简介：在对犬内脏利什曼病（VL）进行诊断评估时，发现 17 只狗中有 2 只同时感染了利什曼原虫（Viannia） braziliensis 和利什曼原虫（Leishmania）chagasi。
方法：进行了特定的聚合酶链反应（PCR）和限制性片段长度多态性-PCR（RFLP-PCR）检测。
结果：对利什曼亚属鉴定的 PCR 检测，随后对皮肤病变和脾脏活检进行 RFLP-PCR 分析，证实了利什曼原虫（Viannia） braziliensis 和利什曼原虫（Leishmania）chagasi 在这些片段中的存在。
结论：本报告强调了在已知两种疾病共存的流行地区，对犬群进行血清学和分子技术进行流行病学监测的重要性。在这种情况下，需要重新评估控制措施。
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