Suzuki K, Morita M, Katoh M, Kidokoro M, Saika S, Yoshizawa S, Hashizume S, Horiuchi K, Okabe N, Shinozaki T
Division of Vaccine Development, Chiba Serum Institute, Japan.
J Med Virol. 1990 Nov;32(3):194-201. doi: 10.1002/jmv.1890320311.
The TD97 strain vaccine virus was prepared from the Tanabe strain measles virus by low-temperature passages in primary cell cultures and ultraviolet (UV) mutagenesis. The TD97 strain exhibited the following characteristics: highly temperature sensitive, neither multiplying nor forming any plaques at 40 degrees C in Vero cells; genetically stable, maintaining high temperature sensitivity after ten successive passages in CE cells at 30 degrees C or 35 degrees C; and M proteins of this virus about 1 KD slower in mobility in SDS-PAGE than that of the Tanabe strain. The TD97 strain was further confirmed to be attenuated by an inoculation test into primate brain. In field trials, 752 healthy children were inoculated with a live virus vaccine prepared with this strain, and the following results were obtained: the seroconversion rate was 97% (517/533), and the average HI antibody titer was 2(5.2). An antibody-increasing effect was also observed in children who were initially seropositive. In children who seroconverted, the rates of fever were 15.7% (55/351) for 37.5 degrees C or higher and 4.0% (14/351) for 39 degrees C or higher. The rash rate was 7.7% (27/351), and the incidence of local reaction was 5.4% (19/351). The TD97 strain is thus considered to be suitable in use for an attenuated measles vaccine.
TD97株疫苗病毒是通过在原代细胞培养物中进行低温传代及紫外线（UV）诱变，由田边株麻疹病毒制备而来。TD97株具有以下特性：高度温度敏感，在Vero细胞中40℃时既不增殖也不形成任何蚀斑；遗传稳定，在CE细胞中于30℃或35℃连续传代十次后仍保持高温敏感性；该病毒的M蛋白在SDS-PAGE中的迁移速度比田边株慢约1KD。通过接种灵长类动物脑内试验进一步证实TD97株已减毒。在现场试验中，752名健康儿童接种了用该毒株制备的活病毒疫苗，获得了以下结果：血清转化率为97%（517/533），平均血凝抑制（HI）抗体滴度为2(5.2)。在最初血清学阳性的儿童中也观察到抗体增强效应。在血清转化的儿童中，体温37.5℃及以上的发热率为15.7%（55/351），体温39℃及以上的发热率为4.0%（14/351）。皮疹率为7.7%（27/351），局部反应发生率为5.4%（19/351）。因此，TD97株被认为适用于减毒麻疹疫苗。
