Fukuda A, Sengün F, Sarpay H E, Konobe T, Saito S, Umino Y, Kohama T
Department of Virus Disease and Vaccine Control, National Institute of Health, Tokyo, Japan.
J Virol Methods. 1996 Sep;61(1-2):1-6. doi: 10.1016/0166-0934(96)02064-2.
Parameters for plaque formation by measles vaccine strains licensed in Japan were studied. For the plaque test, inoculum volume was one of the critical factors for obtaining an appropriate titre of the sample. A linear relationship between the inoculum volume and the apparent reciprocal titre was discovered, enabling the comparison of absolute titres. Another factor affecting the infectivity was the strain-specific temperature sensitivity in the plaque assay. Although all the vaccine strains tested showed the highest titre at 35 degrees C, the pattern of the temperature sensitivity differed from one strain to another. These factors must be taken into consideration in order to obtain an appropriate titre of a vaccine virus.
对日本批准的麻疹疫苗株形成蚀斑的参数进行了研究。对于蚀斑试验，接种量是获得样品适当滴度的关键因素之一。发现接种量与表观倒数滴度之间存在线性关系，从而能够比较绝对滴度。影响感染性的另一个因素是蚀斑试验中菌株特异性的温度敏感性。虽然所有测试的疫苗株在35摄氏度时滴度最高，但温度敏感性模式因菌株而异。为了获得疫苗病毒的适当滴度，必须考虑这些因素。
