Ponomareva E G, Emelyanova N V, Nikitina V E
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
Bull Exp Biol Med. 2012 Jan;152(3):333-5. doi: 10.1007/s10517-012-1521-2.
We studied the effect of fucose-specific lectin from Azospirillum brasilense Sp7 bacteria and Phaseolus vulgarus phytohemagglutinin (PHA) on activity of IFN-γ, IFN-α, IL-1α, IL-4, IL-8, and TNF-α in human blood in vitro. During the experiment, IFN-γ production increased from 25 pg/ml in controls to 103 and 56 pg/ml under the influence of lectin and PHA, respectively. Agglutinins had similar effects on IFN-α production. Bacterial lectin increased IL-1α level from 2.65±0.08 to 8.45±0.41 pg/ml. PHA also increased IL-1α levels, but this effect was by 1.5-2 times less pronounced. Bacterial lectin and PHA increased production IL-4 by almost 2 times and production of IL-8 by 6 and 5 times, respectively, compared to the control. No differences were found in the effects of bacterial lectin and PHA on TNF-α synthesis. Experiments showed that immunostimulatory and immunomodulatory effects of bacterial lectin are more pronounced than those of PHA.
我们研究了来自巴西固氮螺菌Sp7细菌的岩藻糖特异性凝集素和菜豆植物血凝素（PHA）对体外人血中IFN-γ、IFN-α、IL-1α、IL-4、IL-8和TNF-α活性的影响。在实验过程中，IFN-γ的产生量从对照组的25 pg/ml分别增加到在凝集素和PHA作用下的103 pg/ml和56 pg/ml。凝集素对IFN-α的产生有类似作用。细菌凝集素使IL-1α水平从2.65±0.08 pg/ml增加到8.45±0.41 pg/ml。PHA也增加了IL-1α水平，但这种作用的明显程度要低1.5至2倍。与对照组相比，细菌凝集素和PHA分别使IL-4的产生增加了近2倍，IL-8的产生增加了6倍和5倍。未发现细菌凝集素和PHA对TNF-α合成的作用存在差异。实验表明，细菌凝集素的免疫刺激和免疫调节作用比PHA更明显。
