常见和罕见类型非 Merkel 细胞皮肤癌中人类多瘤病毒的流行情况。

Prevalence of human polyomaviruses in common and rare types of non-Merkel cell carcinoma skin cancer.

机构信息

Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791 Bochum, Germany.

出版信息

Br J Dermatol. 2012 Dec;167(6):1315-20. doi: 10.1111/j.1365-2133.2012.11141.x.

DOI:10.1111/j.1365-2133.2012.11141.x

PMID:22803598

Abstract

BACKGROUND

Little is known about the association of human polyomaviruses (HPyVs) other than Merkel cell polyomavirus (MCPyV) with nonmelanoma skin cancer.

OBJECTIVES

To evaluate the presence of HPyV6, HPyV7, trichodysplasia spinulosa-associated polyomavirus (TSV), also called HPyV8, and the recently discovered HPyV9 in basal cell carcinoma (BCC), actinic keratosis (AK), squamous cell carcinoma in situ (SCCis), squamous cell carcinoma (SCC), keratoacanthoma (KA), microcystic adnexal carcinoma (MAC) and atypical fibroxanthoma (AFX).

METHODS

Archival paraffin-embedded samples (n = 193: 41 BCC, 31 AK, 8 SCCis, 52 SCC, 42 KA, 5 MAC and 14 AFX) were analysed for the presence of the respective HPyV by polymerase chain reaction (PCR). HPyV DNA loads (HPyV DNA copies per β-globin gene copy) were determined in all HPyV-positive samples by quantitative real-time PCR. Immunohistochemical analysis of MCPyV large T-antigen (LTA) expression was performed using the monoclonal antibody CM2B4.

RESULTS

MCPyV DNA was found in 29% of BCC, 19% of AK, 25% of SCCis, 27% of SCC, 29% of KA, 0% of MAC and 29% of AFX. MCPyV DNA loads never exceeded 0·3 MCPyV DNA copies per β-globin gene copy (median 0·004). In the immunohistochemical analysis of MCPyV LTA expression, all evaluated samples (32 MCPyV DNA-positive samples) were LTA negative. HPyV6 DNA was found in 7% of BCC, 3% of AK, 12% of SCCis, 4% of SCC, 5% of KA, and 0% of MAC and AFX. HPyV6 DNA loads never exceeded 0·7 HPyV6 DNA copies per β-globin gene copy (median 0·015). None of the 193 samples was positive for HPyV7, TSV or HPyV9 DNA.

CONCLUSIONS

Our findings argue against a pathogenic role for MCPyV, HPyV6, HPyV7, TSV and HPyV9 in the analysed types of non-Merkel cell carcinoma skin cancer.

摘要

背景

除 Merkel 细胞多瘤病毒（MCPyV）外，人们对其他人类多瘤病毒（HPyV）与非黑色素瘤皮肤癌之间的关系知之甚少。

目的

评估 HPyV6、HPyV7、毛发性丘疹性发疹相关多瘤病毒（TSV），也称为 HPyV8，以及最近发现的 HPyV9 在基底细胞癌（BCC）、光化性角化病（AK）、原位鳞状细胞癌（SCCis）、鳞状细胞癌（SCC）、角化棘皮瘤（KA）、微囊性附件癌（MAC）和非典型纤维黄色瘤（AFX）中的存在情况。

方法

对 193 例存档石蜡包埋样本（41 例 BCC、31 例 AK、8 例 SCCis、52 例 SCC、42 例 KA、5 例 MAC 和 14 例 AFX）进行聚合酶链反应（PCR）检测以确定各自的 HPyV 存在情况。通过定量实时 PCR 确定所有 HPyV 阳性样本中的 HPyV DNA 载量（每β-球蛋白基因拷贝的 HPyV DNA 拷贝数）。使用单克隆抗体 CM2B4 对 MCPyV 大 T 抗原（LTA）表达进行免疫组织化学分析。

结果

29%的 BCC、19%的 AK、25%的 SCCis、27%的 SCC、29%的 KA、0%的 MAC 和 29%的 AFX 中发现了 MCPyV DNA。MCPyV DNA 载量从未超过 0.3 MCPyV DNA 拷贝/β-球蛋白基因拷贝（中位数 0.004）。在 MCPyV LTA 表达的免疫组织化学分析中，所有评估的样本（32 个 MCPyV DNA 阳性样本）均为 LTA 阴性。7%的 BCC、3%的 AK、12%的 SCCis、4%的 SCC、5%的 KA 和 0%的 MAC 和 AFX 中发现了 HPyV6 DNA。HPyV6 DNA 载量从未超过 0.7 HPyV6 DNA 拷贝/β-球蛋白基因拷贝（中位数 0.015）。193 个样本均未检测到 HPyV7、TSV 或 HPyV9 DNA 阳性。