Research center for cell fate control, College of Pharmacy, Sookmyung Women's University , Seoul , Republic of Korea.
J Enzyme Inhib Med Chem. 2013 Oct;28(5):954-9. doi: 10.3109/14756366.2012.697057. Epub 2012 Jul 18.
Microglial activation has been associated with neurodegenerative diseases by inducing the neuroinflammatory mediators such as nitric oxide (NO), TNF-α and IL-1β. (-)-Nyasol, a norlignan isolated from a medicinal plant Anemarrhena asphodeloides, showed anti-inflammatory potential in lipopolysaccharide (LPS)-activated BV-2 microglial cells. (-)-Nyasol inhibited the production of NO and prostaglandin E2 (PGE2) and also the expression of inducible nitric oxide synthase and cyclooxygenase-2, which are responsible for the respective production of NO and PGE2. It also suppressed the mRNA levels of TNF-α and IL-1β in activated microglial cells. These effects of (-)-nyasol were correlated with the inactivation of p38 MAPK and the suppression of LPS-induced I-κBα degradation. Taken together, these results suggest that (-)-nyasol can be a modulator in neuroinflammatory conditions induced by microglial activation.
小胶质细胞激活通过诱导一氧化氮 (NO)、TNF-α 和 IL-1β 等神经炎症介质与神经退行性疾病有关。(-)-Nyasol 是从药用植物知母中分离得到的一种木脂素，在脂多糖 (LPS) 激活的 BV-2 小胶质细胞中显示出抗炎潜力。(-)-Nyasol 抑制了 NO 和前列腺素 E2 (PGE2)的产生，也抑制了诱导型一氧化氮合酶和环氧化酶-2 的表达，这两种酶分别负责 NO 和 PGE2 的产生。它还抑制了活化小胶质细胞中 TNF-α 和 IL-1β 的 mRNA 水平。(-)-nyasol 的这些作用与 p38 MAPK 的失活和 LPS 诱导的 I-κBα 降解的抑制有关。总之，这些结果表明 (-)-nyasol 可以作为小胶质细胞激活引起的神经炎症状态的调节剂。
