UMR Inserm U665, Pointe-à-Pitre, Guadeloupe, France.
Transfusion. 2013 Mar;53(3):627-36. doi: 10.1111/j.1537-2995.2012.03779.x. Epub 2012 Jul 15.
The recent in vitro demonstration that inositol hexaphosphate-loaded red blood cells (IHP-RBCs) may reduce the risks of sickling of sickle RBCs (SS RBCs) exposed to hypoxia make these modified RBCs potentially useful in transfused sickle cell anemia (SCA) patients.
Hemorheologic properties of IHP-RBCs, normal RBCs (AA RBCs), SS RBCs, SS RBCs plus AA RBCs, and SS RBCs plus IHP-RBCs were compared under normoxia and/or after hypoxic challenges.
Although IHP-RBCs have reduced deformability compared with SS RBCs or AA RBCs, IHP-RBCs exhibited lower aggregability than AA RBCs and SS RBCs and, when mixed with SS RBCs, the aggregation level was below the one of SS RBCs alone or SS RBCs plus AA RBCs. Blood viscosity of SS RBC plus IHP-RBC suspension was lower than the viscosity of SS RBCs alone and greater than viscosity of SS RBCs plus AA RBCs. The hypoxic challenge was detrimental for deformability and viscosity of SS RBCs alone or SS plus AA RBC suspension but not for SS plus IHP-RBC suspension.
Our results support the fact that IHP-RBCs could be useful in SCA by decreasing RBC aggregation and blunting the adverse effects of hypoxia on RBC deformability and blood viscosity.
最近的体外研究表明，六磷酸肌醇负载的红细胞（IHP-RBC）可以降低缺氧条件下镰状红细胞（SS RBC）镰变的风险，使这些改良的 RBC 在输注镰状细胞贫血（SCA）患者中具有潜在的用途。
在常氧和/或缺氧挑战下，比较 IHP-RBC、正常 RBC（AA RBC）、SS RBC、SS RBC 加 AA RBC 和 SS RBC 加 IHP-RBC 的血液流变学特性。
虽然 IHP-RBC 的变形性比 SS RBC 或 AA RBC 降低，但 IHP-RBC 的聚集性低于 AA RBC 和 SS RBC，当与 SS RBC 混合时，聚集水平低于 SS RBC 单独或 SS RBC 加 AA RBC。SS RBC 加 IHP-RBC 悬浮液的血液粘度低于 SS RBC 单独的粘度，大于 SS RBC 加 AA RBC 悬浮液的粘度。缺氧挑战对 SS RBC 单独或 SS 加 AA RBC 悬浮液的变形性和粘度有害，但对 SS 加 IHP-RBC 悬浮液没有影响。
我们的结果支持这样一个事实，即 IHP-RBC 可以通过降低 RBC 聚集和减轻缺氧对 RBC 变形性和血液粘度的不利影响，在 SCA 中具有用途。
